ArticleNucleic acids research1997
Plant ribosome shunting in vitro.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Cell-free riboswitches.RSC chemical biology · 2021Review
- Non-canonical translation in RNA viruses.The Journal of general virology · 2012Review
- Competing pathways control host resistance to virus via tRNA modification and programmed ribosomal frameshifting.Molecular systems biology · 2012Article
- Genomic analysis of codon, sequence and structural conservation with selective biochemical-structure mapping reveals highly conserved and dynamic structures in rotavirus RNAs with potential cis-acting functions.Nucleic acids research · 2010Article
- Molecular dissection of the prototype foamy virus (PFV) RNA 5'-UTR identifies essential elements of a ribosomal shunt.Nucleic acids research · 2009Article
- Mechanism of ribosome shunting in Rice tungro bacilliform pararetrovirus.RNA (New York, N.Y.) · 2006Article
- Viral strategies of translation initiation: ribosomal shunt and reinitiation.Progress in nucleic acid research and molecular biology · 2002Review
- The human immunodeficiency virus type 1 gag gene encodes an internal ribosome entry site.Journal of virology · 2001Article
- Termination and peptide release at the upstream open reading frame are required for downstream translation on synthetic shunt-competent mRNA leaders.Molecular and cellular biology · 2000Article
- Leaky scanning is the predominant mechanism for translation of human papillomavirus type 16 E7 oncoprotein from E6/E7 bicistronic mRNA.Journal of virology · 2000Article
- Translational control of viral gene expression in eukaryotes.Microbiology and molecular biology reviews : MMBR · 2000Review
- Ribosome shunting in the cauliflower mosaic virus 35S RNA leader is a special case of reinitiation of translation functioning in plant and animal systems.Genes & development · 2000Article
- Interaction of the cauliflower mosaic virus coat protein with the pregenomic RNA leader.Journal of virology · 2000Article
- A stable hairpin preceded by a short open reading frame promotes nonlinear ribosome migration on a synthetic mRNA leader.RNA (New York, N.Y.) · 1999Article
- Internal ribosome entry sites (IRESs): reality and use.Transgenic research · 1999Review
- Bridging IRES elements in mRNAs to the eukaryotic translation apparatus.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been proposed that cauliflower mosaic virus 35S RNA with its 600 nt long leader uses an unusual translation process (the translational shunt). A wheat germ in vitro translation assay was used to improve the study of this mechanism. Deletions, the introduction of stable stem-loop structures, and the inhibitory effect of antisense oligonucleotides on gene expression were used to determine the roles of various parts of the leader. It was found that the 5'- and 3'-ends of the leader are absolutely required for translation whereas the middle part is apparently dispensable. These results confirm the data already reported from transient expression experiments with protoplasts. However, the in vitro data suggest in contrast to protoplast experiments that only two relatively short regions at both ends, approximately 100 nt each, are required. The in vitro system provides tools for further studying the shunt model at the molecular level and for examining the involvement of proteins in this mechanism. Shunting was also found to occur with the rice tungro bacilliform virus leader. As wheat is neither a host plant of cauliflower mosaic virus nor rice tungro bacilliform virus, the shunt seems to be host independent, a finding that deviates from earlier studies in protoplasts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.