ArticleNucleic acids research1997
Tetracycline-controlled transcription in eukaryotes: novel transactivators with graded transactivation potential.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
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Who cites it
117 citing papers in PubMed, 335 citations in OpenAlex.
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- An intein-split transactivator for intersectional neural imaging and optogenetic manipulation.Nature communications · 2022Article
- Spatial control of gene expression in flies using bacterially derived binary transactivation systems.Insect molecular biology · 2021Article
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- An endoribonuclease-based feedforward controller for decoupling resource-limited genetic modules in mammalian cells.Nature communications · 2020Article
- Erinacine C Activates Transcription from a Consensus ETS DNA Binding Site in Astrocytic Cells in Addition to NGF Induction.Biomolecules · 2020Article
- Characterization and mitigation of gene expression burden in mammalian cells.Nature communications · 2020Article
- Flow-based regenerable chemiluminescence receptor assay for the detection of tetracyclines.Analytical and bioanalytical chemistry · 2020Article
- Externally-Controlled Systems for Immunotherapy: From Bench to Bedside.Frontiers in immunology · 2020Review
- Bronchioalveolar stem cells are a main source for regeneration of distal lung epitheliaThe EMBO journal · 2019Article
- The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human.PLoS genetics · 2019Article
- An improved Tet-on system in microRNA overexpression and CRISPR/Cas9-mediated gene editing.Journal of animal science and biotechnology · 2019Article
- Multiscale effects of heating and cooling on genes and gene networks.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
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- Transcriptional effects of a positive feedback circuit in Drosophila melanogaster.BMC genomics · 2017Article
57 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several tetracycline-controlled transactivators (tTA) were generated which differ in their activation potential by >3 orders of magnitude. The transactivators are fusions between the Tet repressor and minimal transcriptional activation domains derived from Herpes simplex virus protein 16 (VP16). By reducing the VP16 moiety of the previously described tTA to 12 amino acids, potential targets for interactions with various cellular transcription factors were eliminated, as were potential epitopes which may elicit a cellular immune response. When compared with the originally described tTA, these new transactivators are tolerated at higher intracellular concentrations. This will facilitate establishment of tet regulatory systems under a variety of conditions, but particularly when cell type-restricted tetracycline-controlled gene expression is to be achieved in transgenic organisms via homologous recombination.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.