ArticleExperimental & applied acarology1997
Detection of Theileria annulata by the PCR in ticks (Acari:Ixodidae) collected from cattle in Mauritania.
Article in Experimental & applied acarology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 78 citations in OpenAlex.
- Status of babesiosis among domestic herbivores in Iran: a systematic review and meta-analysis.Parasitology research · 2017Pooled it
- Molecular Epidemiology and Genotypic Diversity of Theileria annulata among Bovines Based Upon Tams1 Gene in India.Acta parasitologica · 2025Article
- Article
- Comparative diagnostic performance of recombinant Tams1 protein based dot-ELISA in detecting tropical theileriosis in naturally infected cattle.Scientific reports · 2025Article
- Article
- Epidemiological situation of bovine tropical theileriosis in an arid region in central Tunisia with a phylogenetic analysis of Theileria annulata.Veterinary medicine and science · 2023Article
- Tick-Borne Diseases of Humans and Animals in West Africa.Pathogens (Basel, Switzerland) · 2023Review
- An Update of Evidence for Pathogen Transmission by Ticks of the Genus Hyalomma.Pathogens (Basel, Switzerland) · 2023Article
- Survey of Tick-Borne Zoonotic Agents inTransboundary and emerging diseases · 2023Article
- Development and field evaluation of PCR assays based on minimum lengthFrontiers in veterinary science · 2023Article
- Subcutaneous Ticks in Wild Carnivores: Any Host-Related Differences?Animals : an open access journal from MDPI · 2022Article
- Cultivation, cryopreservation and resuscitation ofFrontiers in veterinary science · 2022Article
- Arthropod-Borne Pathogens in Stray Cats from Northern Italy: A Serological and Molecular Survey.Animals : an open access journal from MDPI · 2020Article
- Clinical and Pathological Studies on Cattle Experimentally Infected withPathogens (Basel, Switzerland) · 2020Article
- A national survey of Ixodidae ticks on privately owned dogs in Italy.Parasites & vectors · 2018Article
- Status of theileriosis among herbivores in Iran: A systematic review and meta-analysis.Veterinary world · 2018Article
- Importance of Common Wall Lizards in the Transmission Dynamics of Tick-Borne Pathogens in the Northern Apennine Mountains, Italy.Microbial ecology · 2017Article
- Molecular and pathological studies on natural cases of bovine theileriosis.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Article
- Detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2016Article
- Survey on infection rate, vectors and molecular identification of Theileria annulata in cattle from North West, Iran.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on the detection of Theileria annulata in infected Hyalomma ticks by the PCR using primers derived from the gene encoding the 30 kDa major merozoite surface antigen (TamsI-1). No inhibition of the PCR was observed and as little as 0.1 pg of parasite DNA, corresponding to 12 sporozoites, could be detected in non-infected tick DNA samples, spiked with T. annulata genomic DNA. Hyalomma dromedarii ticks, fed on a calf experimentally infected with T. annulata, were used to validate the PCR further. The infection rate in the adult ticks, fed as nymphs during the febrile reaction, was high (62%), dropped to zero for 1 day in tick batches that engorged after treatment with Butalex and increased to 30% 2 days later and 38% of the ticks acquired the infection after feeding as nymphs during a carrier state piroplasm parasitaemia of less than 0.1%. As an internal control, 16S tick rDNA sequences could be amplified from T. annulata-negative tick samples. Finally, 202 adult ticks from Mauritania, collected from zebu cattle carrying low levels of Theileria piroplasms, were tested by the PCR. Thirty-eight out of 52 (73%) and 17 out of 30 (57%) H. dromedarii from the Gorgol and Trarza regions, respectively and two out of 30 (7%) Hyalomma marginatum rufipes from the Gorgol region were positive. Hyalomma marginatum rufipes, Rhipicephalus evertsi evertsi and Rhipicephalus guilhoni from the Trarza region were negative. These findings confirm that H. dromedarii is the main vector of T. annulata in Mauritania and that the PCR is a useful method of determining the infection rates in ticks collected from cattle carrying low levels of T. annulata piroplasms.
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