ArticleMolecular biology of the cell1997
An unusual fibrillarin gene and protein: structure and functional implications.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- SHARK: web server for alignment-free homology assessment for intrinsically disordered and unalignable protein regions.Nucleic acids research · 2025Article
- Advances in the structure and function of the nucleolar protein fibrillarin.Frontiers in cell and developmental biology · 2024Review
- FBL promotes cancer cell resistance to DNA damage and BRCA1 transcription via YBX1.EMBO reports · 2023Article
- Analysis ofPharmaceuticals (Basel, Switzerland) · 2023Review
- Article
- Two RNA-binding sites in plant fibrillarin provide interactions with various RNA substrates.Nucleic acids research · 2011Article
- Two GW repeat proteins interact with Tetrahymena thermophila argonaute and promote genome rearrangement.Molecular and cellular biology · 2009Article
- Molecular cloning and gene expression of a fibrillarin homolog of tobacco BY-2 cells.Protoplasma · 2006Article
- Molecular genetic analysis of an SNF2/brahma-related gene in Tetrahymena thermophila suggests roles in growth and nuclear development.Eukaryotic cell · 2006Article
- Unusual features of fibrillarin cDNA and gene structure in Euglena gracilis: evolutionary conservation of core proteins and structural predictions for methylation-guide box C/D snoRNPs throughout the domain Eucarya.Nucleic acids research · 2005Article
- Arginine methylation of recombinant murine fibrillarin by protein arginine methyltransferase.Journal of protein chemistry · 2002Article
- Mutational analysis of fibrillarin and its mobility in living human cells.The Journal of cell biology · 2000Article
- Molecular cloning and targeting of a fibrillarin homolog from Arabidopsis.Plant physiology · 2000Article
- Crystal structure of a fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6 A resolution.The EMBO journal · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diploid germinal nucleus of the ciliated protozoan Tetrahymena thermophila is unusual among eukaryotes in that it encodes a single copy of the gene for rRNA allowing identification of cis-acting mutations in rDNA affecting rRNA structure, function, and processing. The generally conserved nucleolar protein fibrillarin has been characterized from a number of systems and is involved in pre-rRNA processing. We have demonstrated that Tetrahymena has fibrillarin and have analyzed the cDNA and the genomic DNA encoding this protein. The derived amino acid sequence of the N-terminal region of Tetrahymena fibrillarin shows little similarity with the generally highly conserved glycine/arginine-rich N-terminal domain of other eukaryotic fibrillarins. The remainder of the amino acid sequence of the molecule is more conserved. Polyclonal antibodies generated against the full-length Tetrahymena fibrillarin expressed in bacteria recognize a protein of M(r) approximately 32,000 in whole-cell or nucleolar preparations. Immunocytochemistry localizes fibrillarin to nucleoli in the somatic macronuclei of vegetative cells. Transformation experiments demonstrate that fibrillarin is an essential protein in Tetrahymena. The Tetrahymena fibrillarin is expressed but does not complement a NOP1 null mutation when transformed into the yeast Saccharomyces cerevisiae, indicating less functional conservation among fibrillarins than previously suggested.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.