ArticleBiophysical journal1997
Single-channel properties of a rat brain endoplasmic reticulum anion channel.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Modulation of the hepatocyte rough endoplasmic reticulum single chloride channel by nucleotide-Mg2+ interaction.Pflugers Archiv : European journal of physiology · 2012Article
- Mechanisms of U46619-induced contraction of rat pulmonary arteries in the presence and absence of the endothelium.British journal of pharmacology · 2009Article
- Constitutive, translation-independent opening of the protein-conducting channel in the endoplasmic reticulum.Pflugers Archiv : European journal of physiology · 2009Article
- Mechanisms of U46619- and 5-HT-induced contraction of bovine pulmonary arteries: role of chloride ions.British journal of pharmacology · 2007Article
- Redox regulation of CLIC1 by cysteine residues associated with the putative channel pore.Biophysical journal · 2006Article
- A large-conductance anion channel of the Golgi complex.Biophysical journal · 2002Article
- Distinct ion channel classes are expressed on the outer nuclear envelope of T- and B-lymphocyte cell lines.Biophysical journal · 2000Article
- GOLAC: an endogenous anion channel of the Golgi complex.Biophysical journal · 2000Article
- Characterization of inositol-1,4,5-trisphosphate-gated channels in the plasma membrane of rat olfactory neurons.Biophysical journal · 1999Article
- The major outer membrane protein of Chlamydia psittaci functions as a porin-like ion channel.Infection and immunity · 1998Article
- Chloride channel blockers inhibit Ca2+ uptake by the smooth muscle sarcoplasmic reticulum.Biophysical journal · 1998Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many intracellular membranes contain ion channels, although their physiological roles are often poorly understood. In this study we incorporated single anion channels colocalized with rat brain endoplasmic reticulum (ER) ryanodine-sensitive Ca(2+)-release channels into planar lipid bilayers. The channels opened in bursts, with more activity at negative (cytoplasm-ER lumen) membrane potentials, and they occupied four open conductance levels with frequencies well described by the binomial equation. The probability of a protomer being open decreased from approximately 0.7 at -40 mV to approximately 0.2 at +40 mV, and the channels selected between different anions in the order PSCN > PNO3 > PBr > PCl > PF. They were also permeant to cations, including the large cation Tris+ (PTris/PCl = 0.16). Their conductance saturated at 170 pS in choline Cl. The channels were inactivated by 15 microM 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) and blocked with low affinity (KD of 1-100 microM) by anthracene-9-carboxylic acid, ethacrynic acid, frusemide (furosemide), HEPES, the indanyloxyacetic acid derivative IAA-94, 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB), and Zn2+. Unlike protein translocation pores, the channels were unaffected by high salt concentrations or puromycin. They may regulate ER Ca2+ release, or be channel components en route to their final cellular destinations. Alternatively, they may contribute to the fusion machinery involved in intracellular membrane trafficking.
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