ArticleInfection and immunity1997
Cytostatic and cytotoxic effects of activated macrophages and nitric oxide donors on Brugia malayi.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed.
- From Gasotransmitter to Immunomodulator: The Emerging Role of Hydrogen Sulfide in Macrophage Biology.Antioxidants (Basel, Switzerland) · 2023Review
- A mouse infection model and long-term lymphatic endothelium co-culture system to evaluate drugs against adult Brugia malayi.PLoS neglected tropical diseases · 2022Article
- The Immunological Role of Vascular and Lymphatic Endothelial Cells in Filarial Infections.Animals : an open access journal from MDPI · 2022Review
- Structural Requirements for Dihydrobenzoxazepinone Anthelmintics: Actions against Medically Important and Model Parasites:ACS infectious diseases · 2021Article
- Nitric oxide debilitates the neuropathogenic schistosome Trichobilharzia regenti in mice, partly by inhibiting its vital peptidases.Parasites & vectors · 2020Article
- Troponin 1 of human filarial parasite Brugia malayi: cDNA cloning, expression, purification, and its immunoprophylactic potential.Parasitology research · 2019Article
- Leishmania donovani molecules recognized by sera of filaria infected host facilitate filarial infection.Parasitology research · 2018Article
- Polyphenol profile by UHPLC-MS/MS, anti-glycation, antioxidant and cytotoxic activities of several samples of propolis from the northeastern semi-arid region of Brazil.Pharmaceutical biology · 2017Article
- S-Nitrosated Polypropylene Sulfide Nanoparticles for Thiol-Dependent Transnitrosation and Toxicity Against Adult Female Filarial Worms.Advanced healthcare materials · 2015Article
- The M1 and M2 paradigm of macrophage activation: time for reassessment.F1000prime reports · 2014Review
- Cofactor independent phosphoglycerate mutase of Brugia malayi induces a mixed Th1/Th2 type immune response and inhibits larval development in the host.BioMed research international · 2014Article
- Live Brugia malayi microfilariae inhibit transendothelial migration of neutrophils and monocytes.PLoS neglected tropical diseases · 2012Article
- Functional and phenotypic characteristics of alternative activation induced in human monocytes by interleukin-4 or the parasitic nematode Brugia malayi.Infection and immunity · 2011Article
- Characterisation of the Trichinella spiralis deubiquitinating enzyme, TsUCH37, an evolutionarily conserved proteasome interaction partner.PLoS neglected tropical diseases · 2011Article
- Evolution of Th2 immunity: a rapid repair response to tissue destructive pathogens.PLoS pathogens · 2011Article
- Inactivation of the complement anaphylatoxin C5a by secreted products of parasitic nematodes.International journal for parasitology · 2010Article
- Nitric oxide and respiratory helminthic diseases.Journal of biomedicine & biotechnology · 2010Review
- Similarity and diversity in macrophage activation by nematodes, trematodes, and cestodes.Journal of biomedicine & biotechnology · 2010Review
- Alternatively activated and immunoregulatory monocytes in human filarial infections.The Journal of infectious diseases · 2009Article
- A STAT4-dependent Th1 response is required for resistance to the helminth parasite Taenia crassiceps.Infection and immunity · 2004Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The susceptibility of Brugia malayi microfilariae and adults to injury by the murine macrophage cell line J774 activated with gamma interferon and bacterial lipopolysaccharide has been examined in vitro. Parasites of both stages showed a decline in viability over 48 h of coculture with activated macrophages, assessed by their capacity to reduce the tetrazolium salt 3-[4,5-diethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), although adult parasites were more resistant than microfilariae. Removal of parasites to cell-free medium following exposure to activated macrophages for up to 48 h resulted in partial recovery of their capacity to reduce MTT, suggesting that the effects were primarily cytostatic. However, prolonged exposure to activated J774 cells for 72 h resulted in parasite death. Addition of the nitric oxide synthase inhibitor L-NMMA (N(G)-monomethyl-L-arginine monoacetate) indicated that nitric oxide derivatives were responsible for cytostasis and ultimate toxicity. The toxicity of nitric oxide derivatives was confirmed by coincubation of parasites with chemical donors, although far higher concentrations were required than those generated by activated J774 cells, implying additional complexity in macrophage-mediated cytotoxicity. These experiments further suggested that peroxynitrite or its by-products were more potently damaging to filariae than nitric oxide per se. Examination of ultrastructural changes on exposure of parasites to activated macrophages or donors of nitric oxide indicated that hypodermal mitochondria were highly vacuolated, with less prominent cristae. The data are discussed with reference to immunity to lymphatic filariae and their mechanisms of energy generation.
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