ArticleInfection and immunity1997
Increased intracellular survival of Mycobacterium smegmatis containing the Mycobacterium leprae thioredoxin-thioredoxin reductase gene.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
- Mycobacterium tuberculosis Rv1324 Protein Contributes to Mycobacterial Persistence and Causes Pathological Lung Injury in Mice by Inducing Ferroptosis.Microbiology spectrum · 2023Article
- OhrR of Mycobacterium smegmatis senses and responds to intracellular organic hydroperoxide stress.Scientific reports · 2017Article
- Trypanosoma cruzi Differentiates and Multiplies within Chimeric Parasitophorous Vacuoles in Macrophages Coinfected with Leishmania amazonensis.Infection and immunity · 2016Article
- Unique genomic arrangements in an invasive serotype M23 strain of Streptococcus pyogenes identify genes that induce hypervirulence.Journal of bacteriology · 2014Article
- Article
- Inactivation of lsr2 results in a hypermotile phenotype in Mycobacterium smegmatis.Journal of bacteriology · 2008Article
- Thioredoxins in bacteria: functions in oxidative stress response and regulation of thioredoxin genes.Die Naturwissenschaften · 2006Review
- Identification of two Mycobacterium marinum loci that affect interactions with macrophages.Infection and immunity · 2004Article
- Mycobacterium tuberculosis pathogenesis and molecular determinants of virulence.Clinical microbiology reviews · 2003Review
- Identification of two Mycobacterium tuberculosis H37Rv ORFs involved in resistance to killing by human macrophages.BMC microbiology · 2001Article
- Comparative genomics uncovers large tandem chromosomal duplications in Mycobacterium bovis BCG Pasteur.Yeast (Chichester, England) · 2000Article
- Identification of a Mycobacterium tuberculosis gene that enhances mycobacterial survival in macrophages.Journal of bacteriology · 2000Article
- [Expression of selenoproteins in monocytes and macrophages--implications for the immune system].Medizinische Klinik (Munich, Germany : 1983) · 1999Review
- Mycobacterium tuberculosis catalase and peroxidase activities and resistance to oxidative killing in human monocytes in vitro.Infection and immunity · 1999Article
- sigmaR, an RNA polymerase sigma factor that modulates expression of the thioredoxin system in response to oxidative stress in Streptomyces coelicolor A3(2).The EMBO journal · 1998Article
- Thioredoxin is an essential protein induced by multiple stresses in Bacillus subtilis.Journal of bacteriology · 1998Article
- Identification and characterization of a novel extracellular ferric reductase from Mycobacterium paratuberculosis.Infection and immunity · 1998Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The thioredoxin (Trx) system of Mycobacterium leprae is expressed as a single hybrid protein containing thioredoxin reductase (TR) at its N terminus and Trx at its C terminus. This hybrid Trx system is unique to M. leprae, since in all other organisms studied to date, including other mycobacteria, both TR and Trx are expressed as two separate proteins. Because Trx has been shown to scavenge reactive oxygen species, we have investigated whether the TR-Trx gene product can inhibit oxygen-dependent killing of mycobacteria by human mononuclear phagocytes and as such could contribute to mycobacterial virulence. The gene encoding M. leprae TR-Trx was cloned into the apathogenic, fast-growing bacterium Mycobacterium smegmatis. Recombinant M. smegmatis containing the gene encoding TR-Trx was killed to a significantly lesser extent than M. smegmatis containing the identical vector with either no insert or a control M. leprae construct unrelated to TR-Trx. Upon phagocytosis, M. smegmatis was shown to be killed predominantly by oxygen-dependent macrophage-killing mechanisms. Coinfection of M. smegmatis expressing the gene encoding TR-Trx together with Staphylococcus aureus, which is known to be killed via oxygen-dependent microbicidal mechanisms, revealed that the TR-Trx gene product interferes with the intracellular killing of this bacterium. A similar coinfection with Streptococcus pyogenes, known to be killed by oxygen-independent mechanisms, showed that the TR-Trx gene product did not influence the oxygen-independent killing pathway. The data obtained in this study suggest that the Trx system of M. leprae can inhibit oxygen-dependent killing of intracellular bacteria and thus may represent one of the mechanisms by which M. leprae can deal with oxidative stress within human mononuclear phagocytes.
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