ArticleInfection and immunity1997
The tumor necrosis factor alpha-stimulating region of galactose-inhibitable lectin of Entamoeba histolytica activates gamma interferon-primed macrophages for amebicidal activity mediated by nitric oxide.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 75 citations in OpenAlex.
- Entamoeba histolytica extracellular vesicles drive pro-inflammatory monocyte signaling.PLoS neglected tropical diseases · 2025Article
- Sex Difference in Amebiasis.Current topics in microbiology and immunology · 2023Article
- Entamoeba histolytica activation of caspase-1 degrades cullin that attenuates NF-κB dependent signaling from macrophages.PLoS pathogens · 2021Article
- Monocyte dysregulation: consequences for hepatic infections.Seminars in immunopathology · 2021Review
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- Article
- Entamoeba histolytica: Host parasite interactions at the colonic epithelium.Tissue barriers · 2017Article
- Investigating the Role of TNF-α and IFN-γ Activation on the Dynamics of iNOS Gene Expression in LPS Stimulated Macrophages.PloS one · 2016Article
- The effect of IFN-γ and TGF-β in the functional activity of mononuclear cells in the presence of Entamoeba histolytica.Parasites & vectors · 2015Article
- Entamoeba histolytica: adhesins and lectins in the trophozoite surface.Molecules (Basel, Switzerland) · 2015Review
- Immune markers characteristic for asymptomatically infected and diseased Entamoeba histolytica individuals and their relation to sex.BMC infectious diseases · 2014Article
- Gal-lectin-dependent contact activates the inflammasome by invasive Entamoeba histolytica.Mucosal immunology · 2014Article
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- Proteases from Entamoeba spp. and Pathogenic Free-Living Amoebae as Virulence Factors.Journal of tropical medicine · 2013Article
- The dynamic interdependence of amebiasis, innate immunity, and undernutrition.Seminars in immunopathology · 2012Review
- CD4+ and CD8+ T cell- and IL-17-mediated protection against Entamoeba histolytica induced by a recombinant vaccine.Vaccine · 2011Article
- Association between TNF-alpha and Entamoeba histolytica diarrhea.The American journal of tropical medicine and hygiene · 2010Article
- Protection against intestinal amebiasis by a recombinant vaccine is transferable by T cells and mediated by gamma interferon.Infection and immunity · 2009Article
- Host-microbe interactions and defense mechanisms in the development of amoebic liver abscesses.Clinical microbiology reviews · 2009Review
- Characterization of Entamoeba histolytica intermediate subunit lectin-specific human monoclonal antibodies generated in transgenic mice expressing human immunoglobulin loci.Infection and immunity · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Entamoeba histolytica adheres via galactose-lectin (Gal-lectin) to human colonic mucins and intestinal epithelial cells as a prerequisite to amebic invasion. Native Gal-lectin is a protective antigen in the gerbil model of amebiasis. Amino acids 596 to 1082 of Gal-lectin mediate E. histolytica adherence to target cells and stimulate tumor necrosis factor alpha (TNF-alpha) production by naive murine bone marrow macrophages (BMM). Resistance to amebiasis requires an effective cell-mediated immune response against E. histolytica trophozoites mediated by nitric oxide (NO) released from activated macrophages. Herein, we determine whether the TNF-alpha-stimulating region of Gal-lectin can activate gamma interferon (IFN-gamma)-primed BMM for NO production and amebicidal activity. Native Gal-lectin (100 to 500 ng/ml) stimulated TNF-alpha and inducible nitric oxide synthase (iNOS) mRNA expression in IFN-gamma-primed BMM as did lipopolysaccharide (100 ng/ml). Primed BMM produced TNF-alpha and NO in response to Gal-lectin in a dose-dependent manner. Antilectin monoclonal antibody IG7, which recognizes a domain (amino acids 596 to 818) of the TNF-alpha mRNA-stimulating region of Gal-lectin, specifically inhibited TNF-alpha and iNOS mRNA induction and TNF-alpha and NO production by primed BMM in response to Gal-lectin (100 ng/ml). Simultaneous treatment of BMM with IFN-gamma and Gal-lectin (100 ng/ml) activated the cells to kill E. histolytica trophozoites, whereas IFN-gamma treatment alone had no effect. In the presence of monoclonal antibody 1G7 or aminoguanidine (an iNOS inhibitor), NO production and amebicidal activity were inhibited >80%. These results suggest that the TNF-alpha-stimulating region of native Gal-lectin is a potent stimulus of IFN-gamma-primed BMM for NO production, which is essential for host defense against amebiasis.
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