ArticleMolecular and cellular biology1997
The composition of coding joints formed in V(D)J recombination is strongly affected by the nucleotide sequence of the coding ends and their relationship to the recombination signal sequences.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- Sequence-dependent dynamics of synthetic and endogenous RSSs in V(D)J recombination.Nucleic acids research · 2020Article
- Genome Topology Control of Antigen Receptor Gene Assembly.Journal of immunology (Baltimore, Md. : 1950) · 2020Review
- On being the right size: antibody repertoire formation in the mouse and human.Immunogenetics · 2018Review
- RUNX1-dependent RAG1 deposition instigates human TCR-δ locus rearrangement.The Journal of experimental medicine · 2014Article
- A role for DNA polymerase mu in the emerging DJH rearrangements of the postgastrulation mouse embryo.Molecular and cellular biology · 2009Article
- A RAG1 mutation found in Omenn syndrome causes coding flank hypersensitivity: a novel mechanism for antigen receptor repertoire restriction.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Evidence of a critical architectural function for the RAG proteins in end processing, protection, and joining in V(D)J recombination.Genes & development · 2002Article
- The RAG proteins in V(D)J recombination: more than just a nuclease.Nucleic acids research · 2001Review
- A C-terminal region of RAG1 contacts the coding DNA during V(D)J recombination.Molecular and cellular biology · 2001Article
- Factors that influence formation of B cell repertoire.Immunologic research · 2000Review
- Mechanistic basis for coding end sequence effects in the initiation of V(D)J recombination.Molecular and cellular biology · 1999Article
- Extensive, nonrandom diversity of excision footprints generated by Ds-like transposon Ascot-1 suggests new parallels with V(D)J recombination.Molecular and cellular biology · 1998Article
- Sequence of the spacer in the recombination signal sequence affects V(D)J rearrangement frequency and correlates with nonrandom Vkappa usage in vivo.The Journal of experimental medicine · 1998Article
- Structure of nonhairpin coding-end DNA breaks in cells undergoing V(D)J recombination.Molecular and cellular biology · 1998Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
V(D)J recombination proceeds in two stages. Precise cleavage at the border of the conserved recombination signal sequences (RSSs) and the coding ends results in flush double-stranded signal ends and coding ends terminating in hairpins. In the second stage, the signal and coding ends are processed into signal and coding joints. Coding ends containing certain nucleotide homopolymers affect the efficiency of V(D)J recombination. In this study, we have tested the effect of small changes in coding-end nucleotide composition on the frequency of coding- and signal joint formation. Furthermore, we have determined the sequences of coding joints resulting from recombination of coding ends with different compositions. We found that the presence of two T nucleotides 5' of both RSSs, but not a single T, reduces the frequency of signal joint formation, i.e., interferes with the cleavage stage of V(D)J recombination. However, coding-joint processing is sensitive even to a single T. Both the sequence of the coding ends and the particular RSS (12-mer or 23-mer) with which the coding end is associated affect the final composition of the coding joints. Thus, the presence of P nucleotides, the conservation of one undeleted coding end, the formation of joints without any deletions, and the template-dependent insertion of nucleotides are strongly influenced by the coding-end nucleotide composition and/or RSS association. The implications of these results with respect to the processing of coding ends are discussed.
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