ArticleMolecular and cellular biology1997
The rRNA-processing function of the yeast U14 small nucleolar RNA can be rescued by a conserved RNA helicase-like protein.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- The Efg1-Bud22 dimer associates with the U14 snoRNP contacting the 5' rRNA domain of an early 90S pre-ribosomal particle.Nucleic acids research · 2024Article
- RNA folding and functions of RNA helicases in ribosome biogenesis.RNA biology · 2022Review
- Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast.Nature communications · 2021Article
- DEAD-box RNA helicase Dbp4/DDX10 is an enhancer of α-synuclein toxicity and oligomerization.PLoS genetics · 2021Article
- Stepwise and dynamic assembly of the earliest precursors of small ribosomal subunits in yeast.Genes & development · 2016Article
- DEAD-box RNA helicase Dbp4 is required for small-subunit processome formation and function.Molecular and cellular biology · 2015Article
- Nucleolar proteins Bfr2 and Enp2 interact with DEAD-box RNA helicase Dbp4 in two different complexes.Nucleic acids research · 2014Article
- Ribosome biogenesis in the yeast Saccharomyces cerevisiae.Genetics · 2013Review
- DExD/H-box RNA helicases in ribosome biogenesis.RNA biology · 2013Review
- Clustered organization, polycistronic transcription, and evolution of modification-guide snoRNA genes in Euglena gracilis.Molecular genetics and genomics : MGG · 2012Article
- Gene regulation in Giardia lambia involves a putative microRNA derived from a small nucleolar RNA.PLoS neglected tropical diseases · 2011Article
- Effects of the NUP98-DDX10 oncogene on primary human CD34+ cells: role of a conserved helicase motif.Leukemia · 2010Article
- Powering through ribosome assembly.RNA (New York, N.Y.) · 2009Review
- Identification of protein binding sites on U3 snoRNA and pre-rRNA by UV cross-linking and high-throughput analysis of cDNAs.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- A novel small-subunit processome assembly intermediate that contains the U3 snoRNP, nucleolin, RRP5, and DBP4.Molecular and cellular biology · 2009Article
- Differential RNA-dependent ATPase activities of four rRNA processing yeast DEAD-box proteins.Biochemistry · 2008Article
- The helicase Has1p is required for snoRNA release from pre-rRNA.Molecular and cellular biology · 2006Article
- Comprehensive mutational analysis of yeast DEXD/H box RNA helicases required for small ribosomal subunit synthesis.Molecular and cellular biology · 2006Article
- Role of RNA helicases in HIV-1 replication.Nucleic acids research · 2006Review
- Dead-box proteins: a family affair--active and passive players in RNP-remodeling.Nucleic acids research · 2006Review
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3 authors.
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Abstract
The phylogenetically conserved U14 small nucleolar RNA is required for processing of rRNA, and this function involves base pairing with conserved complementary sequences in 18S RNA. With a view to identifying other important U14 interactions, a stem-loop domain required for activity of Saccharomyces cerevisiae U14 RNAs (the Y domain) was first subjected to detailed mutational analysis. The mapping results showed that most nucleotides of the Y domain can be replaced without affecting function, except for loop nucleotides conserved among five different yeast species. Defective variants were then used to identify both intragenic and extragenic suppressor mutations. All of the intragenic mutations mapped within six nucleotides of the primary mutation, suggesting that suppression involves a change in conformation and that the loop element is involved in an essential intermolecular interaction rather than intramolecular base pairing. A high-copy extragenic suppressor gene, designated DBP4 (DEAD box protein 4), encodes an essential, putative RNA helicase of the DEAD-DEXH box family. Suppression by DBP4 (initially CA4 [T.-H. Chang, J. Arenas, and J. Abelson, Proc. Natl. Acad. Sci. USA 87:1571-1575, 1990]) restores the level of 18S rRNA and is specific for the Y domain but is not allele specific. DBP4 is predicted to function either in assembly of the U14 small nucleolar RNP or, more likely, in its interaction with other components of the rRNA processing apparatus. Mediating the interaction of U14 with precursor 18S RNA is an especially attractive possibility.
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