ArticleMolecular and cellular biology1997
Localization and posttranslational modifications of otefin, a protein required for vesicle attachment to chromatin, during Drosophila melanogaster development.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- Developmental changes in nuclear lamina components during germ cell differentiation.Nucleus (Austin, Tex.) · 2024Article
- PIGB maintains nuclear lamina organization in skeletal muscle of Drosophila.The Journal of cell biology · 2024Article
- Interactions of Chromatin with the Nuclear Lamina and Nuclear Pore Complexes.International journal of molecular sciences · 2023Review
- Identification of PP2A-B55 targets uncovers regulation of emerin during nuclear envelope reassembly inOpen biology · 2023Article
- ESCRT-III-mediated membrane fusion drives chromosome fragments through nuclear envelope channels.The Journal of cell biology · 2020Article
- Nuclear lamina dysfunction triggers a germline stem cell checkpoint.Nature communications · 2018Article
- Maternal Proteins That Are Phosphoregulated upon Egg Activation Include Crucial Factors for Oogenesis, Egg Activation and Embryogenesis inG3 (Bethesda, Md.) · 2018Article
- Invertebrate models of lamin diseases.Nucleus (Austin, Tex.) · 2018Review
- Laminopathies: what can humans learn from fruit flies.Cellular & molecular biology letters · 2018Review
- Article
- The Drosophila nuclear lamina protein otefin is required for germline stem cell survival.Developmental cell · 2013Article
- Protein phosphorylation changes reveal new candidates in the regulation of egg activation and early embryogenesis in D. melanogaster.Developmental biology · 2012Article
- Functional analysis of centrosomal kinase substrates in Drosophila melanogaster reveals a new function of the nuclear envelope component otefin in cell cycle progression.Molecular and cellular biology · 2012Article
- The different function of single phosphorylation sites of Drosophila melanogaster lamin Dm and lamin C.PloS one · 2012Article
- Tissue-specific defects are caused by loss of the Drosophila MAN1 LEM domain protein.Genetics · 2008Article
- NHK-1 phosphorylates BAF to allow karyosome formation in the Drosophila oocyte nucleus.The Journal of cell biology · 2007Article
- Article
- Essential roles for Caenorhabditis elegans lamin gene in nuclear organization, cell cycle progression, and spatial organization of nuclear pore complexes.Molecular biology of the cell · 2000Article
- Temporal differences in the appearance of NEP-B78 and an LBR-like protein during Xenopus nuclear envelope reassembly reflect the ordered recruitment of functionally discrete vesicle types.The Journal of cell biology · 1999Article
- Detergent-salt resistance of LAP2alpha in interphase nuclei and phosphorylation-dependent association with chromosomes early in nuclear assembly implies functions in nuclear structure dynamics.The EMBO journal · 1998Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Otefin is a peripheral protein of the inner nuclear membrane in Drosophila melanogaster. Here we show that during nuclear assembly in vitro, it is required for the attachment of membrane vesicles to chromatin. With the exception of sperm cells, otefin colocalizes with lamin Dm0 derivatives in situ and presumably in vivo and is present in all somatic cells examined during the different stages of Drosophila development. In the egg chamber, otefin accumulates in the cytoplasm, in the nuclear periphery, and within the nucleoplasm of the oocyte, in a pattern similar to that of lamin Dm0 derivatives. There is a relatively large nonnuclear pool of otefin present from stages 6 to 7 of egg chamber maturation through 6 to 8 h of embryonic development at 25 degrees C. In this pool, otefin is peripherally associated with a fraction containing the membrane vesicles. This association is biochemically different from the association of otefin with the nuclear envelope. Otefin is a phosphoprotein in vivo and is a substrate for in vitro phosphorylation by cdc2 kinase and cyclic AMP-dependent protein kinase. A major site for cdc2 kinase phosphorylation in vitro was mapped to serine 36 of otefin. Together, our data suggest an essential role for otefin in the assembly of the Drosophila nuclear envelope.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.