ArticleMolecular and cellular biology1997
Shc contains two Grb2 binding sites needed for efficient formation of complexes with SOS in B lymphocytes.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 93 citations in OpenAlex.
- The Configuration of GRB2 in Protein Interaction and Signal Transduction.Biomolecules · 2024Review
- Role of c-Src in Carcinogenesis and Drug Resistance.Cancers · 2023Review
- Cell cycle control by the insulin-like growth factor signal: at the crossroad between cell growth and mitotic regulation.Cell cycle (Georgetown, Tex.) · 2023Review
- Accessory proteins of the RAS-MAPK pathway: moving from the side line to the front line.Communications biology · 2021Review
- Article
- Dihydrotestosterone potentiates EGF-induced ERK activation by inducing SRC in fetal lung fibroblasts.American journal of respiratory cell and molecular biology · 2014Article
- Structural and energetic aspects of Grb2-SH2 domain-swapping.Archives of biochemistry and biophysics · 2007Article
- Article
- Disruption of the Shc/Grb2 complex during abelson virus transformation affects proliferation, but not apoptosis.Journal of virology · 2005Article
- Hypermethylation of the spleen tyrosine kinase promoter in T-lineage acute lymphoblastic leukemia.Oncogene · 2003Article
- Discrimination between phosphotyrosine-mediated signaling properties of conventional and neuronal Shc adapter molecules.Oncogene · 2002Article
- Spleen tyrosine kinase (Syk) deficiency in childhood pro-B cell acute lymphoblastic leukemia.Oncogene · 2001Article
- PTP-PEST, a scaffold protein tyrosine phosphatase, negatively regulates lymphocyte activation by targeting a unique set of substrates.The EMBO journal · 2001Article
- Constitutive activation of the Ras/MAP kinase pathway and enhanced TCR signaling by targeting the Shc adaptor to membrane rafts.Oncogene · 2000Article
- Accelerated mammary tumor development in mutant polyomavirus middle T transgenic mice expressing elevated levels of either the Shc or Grb2 adapter protein.Molecular and cellular biology · 1999Article
- Signal transduction by beta1 integrin receptors in human chondrocytes in vitro: collaboration with the insulin-like growth factor-I receptor.The Biochemical journal · 1999Article
- Cloning and characterization of mPAL, a novel Shc SH2 domain-binding protein expressed in proliferating cells.Oncogene · 1999Article
- Role of tyrosine residues and protein interaction domains of SHC adaptor in VEGF receptor 3 signaling.Oncogene · 1999Article
- Negative signaling in health and disease.Immunologic research · 1999Review
- Regulation of RasGRP via a phorbol ester-responsive C1 domain.Molecular and cellular biology · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Cross-linking of the B-cell antigen receptor (BCR) induces tyrosine phosphorylation of Shc, which is believed to lead to the activation of Ras. Previous work has shown that tyrosine-phosphorylated Shc forms complexes with another adapter protein, Grb2, and the Ras guanine nucleotide exchange factor SOS. Here, we demonstrate that phosphorylation of Shc by the hematopoietic cell-specific tyrosine kinase Syk induces binding of Grb2 to Shc, suggesting that Syk phosphorylates Shc in stimulated B cells. Surprisingly, Syk-phosphorylated Shc possesses two Grb2 binding sites rather than the one site that has been previously reported. Both of these sites are required for efficient formation of Shc-Grb2-SOS complexes in vitro and in vivo. We suggest that two Grb2 proteins anchored by a single Shc protein bind simultaneously to one SOS molecule, resulting in a complex that is more stable than a complex containing only a single Grb2 protein bound to one SOS molecule. This model is consistent with our observation that BCR stimulation greatly increases the amount of SOS associated with Grb2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.