ArticleMolecular and cellular biology1997
Precise switching of DNA replication timing in the GC content transition area in the human major histocompatibility complex.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 92 citations in OpenAlex.
- A divide-and-conquer approach to uncover the genomic structure of the highly virulent RA strain of Trypanosoma cruzi.Scientific reports · 2025Article
- Compositional Structure of the Genome: A Review.Biology · 2023Review
- Replication Stress, Genomic Instability, and Replication Timing: A Complex Relationship.International journal of molecular sciences · 2021Review
- Cell line differences in replication timing of human glutamate receptor genes and other large genes associated with neural disease.Epigenetics · 2014Article
- Identification of Horizontally-transferred Genomic Islands and Genome Segmentation Points by Using the GC Profile Method.Current genomics · 2014Article
- Replication timing as an epigenetic mark.Epigenetics · 2009Review
- Organization of the amplified type I interferon gene cluster and associated chromosome regions in the interphase nucleus of human osteosarcoma cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- Assignment of isochores for all completely sequenced vertebrate genomes using a consensus.Genome biology · 2008Article
- Pan-S replication patterns and chromosomal domains defined by genome-tiling arrays of ENCODE genomic areas.Genome research · 2007Article
- Article
- Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts.BMC genomics · 2006Article
- Strong regional heterogeneity in base composition evolution on the Drosophila X chromosome.Genetics · 2006Article
- A genome-wide distribution of 8-oxoguanine correlates with the preferred regions for recombination and single nucleotide polymorphism in the human genome.Genome research · 2006Article
- GC/AT-content spikes as genomic punctuation marks.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Isochore structures in the genome of the plant Arabidopsis thaliana.Journal of molecular evolution · 2004Article
- IsoFinder: computational prediction of isochores in genome sequences.Nucleic acids research · 2004Article
- Using analytical ultracentrifugation to study compositional variation in vertebrate genomes.European biophysics journal : EBJ · 2003Article
- In silico chromosome staining: reconstruction of Giemsa bands from the whole human genome sequence.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Molecular and cytological characterization of SspI-family repetitive sequence on the chicken W chromosome.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2002Article
- Genomic anatomy of a premier major histocompatibility complex paralogous region on chromosome 1q21-q22.Genome research · 2001Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
The human genome is composed of long-range G+C% (GC%) mosaic structures thought to be related to chromosome bands. We previously reported a boundary of megabase-sized GC% mosaic domains at the junction area between major histocompatibility complex (MHC) classes II and III, proposing it as a possible chromosome band boundary. DNA replication timing during the S phase is known to be correlated cytogenetically with chromosome band zones, and thus the band boundaries have been predicted to contain a switch point for DNA replication timing. In this study, to identify to the nucleotide sequence level the replication switch point during the S phase, we determined the precise DNA replication timing for MHC classes II and III, focusing on the junction area. To do this, we used PCR-based quantitation of nascent DNA obtained from synchronized human myeloid leukemia HL60 cells. The replication timing changed precisely in the boundary region with a 2-h difference between the two sides, supporting the prediction that this region may be a chromosome band boundary. We supposed that replication fork movement terminates (pauses) or significantly slows in the switch region, which contains dense Alu clusters; polypurine/polypyrimidine tracts; di-, tri-, or tetranucleotide repeats; and medium-reiteration-frequency sequences. Because the nascent DNA in the switch region was recovered at low efficiency, we investigated whether this region is associated with the nuclear scaffold and found three scaffold-associated regions in and around the switch region.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.