ArticleMolecular and cellular biology1997
Small heat shock protein suppression of Vpr-induced cytoskeletal defects in budding yeast.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- The SAGA complex, together with transcription factors and the endocytic protein Rvs167p, coordinates the reprofiling of gene expression in response to changes in sterol composition inMolecular biology of the cell · 2017Article
- Critical role for heat shock protein 20 (HSP20) in migration of malarial sporozoites.The Journal of biological chemistry · 2012Article
- Hsp42 is required for sequestration of protein aggregates into deposition sites in Saccharomyces cerevisiae.The Journal of cell biology · 2011Article
- HIV-1 viral protein R (Vpr) and its interactions with host cell.Current HIV research · 2009Review
- HIV-1 Vpr inhibits cytokinesis in human proximal tubule cells.Kidney international · 2008Article
- HIV-1 Vpr: mechanisms of G2 arrest and apoptosis.Experimental and molecular pathology · 2008Review
- The heterologous overexpression of hsp23, a small heat-shock protein gene from Trichoderma virens, confers thermotolerance to T. harzianum.Current genetics · 2007Article
- Antagonistic interaction of HIV-1 Vpr with Hsf-mediated cellular heat shock response and Hsp16 in fission yeast (Schizosaccharomyces pombe).Retrovirology · 2007Article
- Analysis of HIV-1 Vpr determinants responsible for cell growth arrest in Saccharomyces cerevisiae.Retrovirology · 2004Article
- Subcellular localization of the yeast proteome.Genes & development · 2002Article
- Alpha-crystallin-type heat shock proteins: socializing minichaperones in the context of a multichaperone network.Microbiology and molecular biology reviews : MMBR · 2002Review
- HIV-1 rev depolymerizes microtubules to form stable bilayered rings.The Journal of cell biology · 2000Article
- HIV-1 Vpr interacts with the nuclear transport pathway to promote macrophage infection.Genes & development · 1998Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Expression of the auxiliary human immunodeficiency virus type 1 (HIV-1) protein Vpr causes arrest of primate host cells in G2. Expression of this protein in budding yeast has been previously reported to cause growth arrest and a large-cell phenotype. Investigation of the effect of Vpr expression in budding yeast, reported here, showed that it causes disruption of the actin cytoskeleton. Expression of HSP42, the gene for a small heat shock protein (sHSP), from a high-copy-number plasmid reversed this effect. The sHSPs are induced by exposure of cells to thermal, osmotic, and oxidative stresses and to mitogens. In animal cells, overexpression of sHSPs causes increased resistance to stress and stabilization of actin stress fibers. Yeast cells subjected to mild stress, such as shifting from 23 to 39 degrees C, arrest growth and then resume cell division. Growth arrest is accompanied by transient disorganization of the cytoskeleton. Yeast in which the HSP42 gene was disrupted and which was subjected to moderate thermal stress reorganized the actin cytoskeleton more slowly than did wild-type control cells. These results demonstrate that in yeast, as in metazoan cells, sHSPs promote maintenance of the actin cytoskeleton.
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Registered trials
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