ArticleMolecular and cellular biology1997
Interdigitated residues within a small region of VP16 interact with Oct-1, HCF, and DNA.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 49 citations in OpenAlex.
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- Aberrant RNA polymerase initiation and processivity on the genome of a herpes simplex virus 1 mutant lacking ICP27.Journal of virology · 2024Article
- Regulation of alphaherpesvirus protein via post-translational phosphorylation.Veterinary research · 2022Review
- Duck Plague Virus pUL48 Protein Activates the Immediate-Early Gene to Initiate the Transcription of the Virus Gene.Frontiers in microbiology · 2021Article
- ICP22/IE63 Mediated Transcriptional Regulation and Immune Evasion: Two Important Survival Strategies for Alphaherpesviruses.Frontiers in immunology · 2021Review
- RNA Polymerase II Promoter-Proximal Pausing and Release to Elongation Are Key Steps Regulating Herpes Simplex Virus 1 Transcription.Journal of virology · 2020Article
- The Role of VP16 in the Life Cycle of Alphaherpesviruses.Frontiers in microbiology · 2020Review
- De Novo Herpes Simplex Virus VP16 Expression Gates a Dynamic Programmatic Transition and Sets the Latent/Lytic Balance during Acute Infection in Trigeminal Ganglia.PLoS pathogens · 2016Article
- Analysis of the interaction between the essential herpes simplex virus 1 tegument proteins VP16 and VP1/2.Journal of virology · 2012Article
- Structure and VP16 binding of the Mediator Med25 activator interaction domain.Nature structural & molecular biology · 2011Article
- Therapeutic implications of new insights into the critical role of VP16 in initiating the earliest stages of HSV reactivation from latency.Future medicinal chemistry · 2010Article
- The latency-associated nuclear antigen interacts with MeCP2 and nucleosomes through separate domains.Journal of virology · 2010Article
- Article
- Sequence and function of canine herpesvirus alpha-transinducing factor and its interaction with an immediate early promoter.Virus genes · 2006Article
- Varicella-zoster virus open reading frame 10 is a virulence determinant in skin cells but not in T cells in vivo.Journal of virology · 2006Article
- Article
- The reading frame BPLF1 of Epstein-Barr virus: a homologue of herpes simplex virus protein VP16.Virus genes · 2004Article
- HCF-1 functions as a coactivator for the zinc finger protein Krox20.The Journal of biological chemistry · 2003Article
- A single amino acid substitution in herpes simplex virus type 1 VP16 inhibits binding to the virion host shutoff protein and is incompatible with virus growth.Journal of virology · 2003Article
- Molecular cloning of Drosophila HCF reveals proteolytic processing and self-association of the encoded protein.Journal of cellular physiology · 2003Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Upon infection, the herpes simplex virus (HSV) activator of immediate-early (IE) gene transcription VP16 forms a multiprotein-DNA complex with two cellular proteins, Oct-1 and HCF. First, VP16 associates with HCF independently of DNA, and this association stimulates subsequent association with Oct-1 on the DNA target of VP16 activation, the TAATGARAT motif found in HSV IE promoters. We have analyzed the involvement of VP16 residues lying near the carboxy-terminal transcriptional activation domain of VP16 in associating with HCF, Oct-1, and DNA. To assay VP16 association with HCF, we developed an electrophoretic mobility retardation assay in which HCF is used to retard the mobility of a hybrid VP16-GAL4 DNA-binding domain fusion protein bound to a GAL4 DNA-binding site. Analysis of an extensive set of individual and combined alanine substitutions over a 61-amino-acid region of VP16 shows that, even within a region as small as 13 amino acids, there are separate residues involved in association with either HCF, DNA, or Oct-1 bound to DNA; indeed, of two immediately adjacent amino acids in VP16, one is important for DNA binding and the other is important for HCF binding. These results suggest that a small region in VP16 is important for linking in close juxtaposition the four components of the VP16-induced complex and support the hypothesis that the structure of the Oct-1-VP16 interaction in this complex is similar to that formed by the yeast transcriptional regulatory proteins MATa1 and MAT alpha2. We propose that HCF stabilizes this Oct-1-VP16 interaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.