ArticleMolecular and cellular biology1997
Adhesion-dependent regulation of an A+U-rich element-binding activity associated with AUF1.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 144 citations in OpenAlex.
- Article
- Interleukin-1 Beta-A Friend or Foe in Malignancies?International journal of molecular sciences · 2018Review
- Adrenergic stimulation alters the expression of inflammasome components and interleukins in primary human monocytes.Experimental and therapeutic medicine · 2016Article
- Interleukin-17 enhances immunosuppression by mesenchymal stem cells.Cell death and differentiation · 2014Article
- Sustained stabilization of Interleukin-8 mRNA in human macrophages.RNA biology · 2014Article
- Pro-neoplastic effects of amphiregulin in colorectal carcinogenesis.Journal of gastrointestinal cancer · 2013Article
- Post-transcriptional regulatory networks in immunity.Immunological reviews · 2013Review
- MKP-1 regulates cytokine mRNA stability through selectively modulation subcellular translocation of AUF1.Cytokine · 2011Article
- RNA-binding protein AUF1 regulates lipopolysaccharide-induced IL10 expression by activating IkappaB kinase complex in monocytes.Molecular and cellular biology · 2011Article
- AUF1 isoform-specific regulation of anti-inflammatory IL10 expression in monocytes.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2008Article
- Chaperone Hsp27, a novel subunit of AUF1 protein complexes, functions in AU-rich element-mediated mRNA decay.Molecular and cellular biology · 2008Article
- FRET-detectable interactions between the ARE binding proteins, HuR and p37AUF1.RNA (New York, N.Y.) · 2007Article
- Article
- Endotoxic shock in AUF1 knockout mice mediated by failure to degrade proinflammatory cytokine mRNAs.Genes & development · 2006Article
- 14-3-3sigma is a p37 AUF1-binding protein that facilitates AUF1 transport and AU-rich mRNA decay.The EMBO journal · 2006Article
- Assembly of AUF1 with eIF4G-poly(A) binding protein complex suggests a translation function in AU-rich mRNA decay.RNA (New York, N.Y.) · 2006Article
- A hairpin-like structure within an AU-rich mRNA-destabilizing element regulates trans-factor binding selectivity and mRNA decay kinetics.The Journal of biological chemistry · 2005Article
- The association of Alu repeats with the generation of potential AU-rich elements (ARE) at 3' untranslated regions.BMC genomics · 2004Article
- Reciprocal regulation of beta-adrenergic receptor mRNA stability by mitogen activated protein kinase activation and inhibition.Molecular and cellular biochemistry · 2004Article
- Roles of AUF1 isoforms, HuR and BRF1 in ARE-dependent mRNA turnover studied by RNA interference.Nucleic acids research · 2004Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Monocyte adherence results in the rapid transcriptional activation and mRNA stabilization of numerous mediators of inflammation and tissue repair. While the enhancer and promoter elements associated with transcriptional activation have been studied, mechanisms linking adhesion, mRNA stabilization, and translation are unknown. GROalpha and interleukin-1beta (IL-1beta) mRNAs are highly labile in nonadhered monocytes but stabilize rapidly after adherence. GROalpha and IL-1beta transcripts both contain A+U-rich elements (AREs) in the 3' untranslated region (UTR) which have been directly associated with rapid mRNA turnover. To determine if the GROalpha ARE region was recognized by factors associated with mRNA degradation, we carried out mobility gel shift analyses using a series of RNA probes encompassing the entire GROalpha transcript. Stable complexes were formed only with the proximal 3' UTR which contained the ARE region. The two slower-moving complexes were rapidly depleted following monocyte adherence but not direct integrin engagement. Deadherence reactivated the two largest ARE-binding complexes and destabilized IL-1beta transcripts. Antibody supershift studies demonstrated that both of these ARE RNA-binding complexes contained AUF1. The formation of these complexes and the accelerated mRNA turnover are phosphorylation-dependent events, as both are induced in adherent monocytes by the tyrosine kinase inhibitor genistein and the p38 MAP kinase inhibitor of IL-1beta translation, SK&F 86002. These results demonstrate that cell adhesion and deadhesion rapidly and reversibly modify both cytokine mRNA stability and the RNA-binding complexes associated with AUF1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.