ArticleMolecular and cellular biology1997
Processing of DNA prior to illegitimate recombination in mouse cells.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Article
- A strand invasion 3' polymerization intermediate of mammalian homologous recombination.Genetics · 2010Article
- Testing predictions of the double-strand break repair model relating to crossing over in Mammalian cells.Genetics · 2004Article
- Genomic DNA double-strand breaks are targets for hepadnaviral DNA integration.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Restriction enzymes increase efficiencies of illegitimate DNA integration but decrease homologous integration in mammalian cells.Nucleic acids research · 2001Article
- Evidence for biased holliday junction cleavage and mismatch repair directed by junction cuts during double-strand-break repair in mammalian cells.Molecular and cellular biology · 2001Article
- The liaison of isotype class switch and mismatch repair: an illegitimate affair.The Journal of experimental medicine · 1999Review
- Exon shuffling mimicked in cell culture.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Hex1: a new human Rad2 nuclease family member with homology to yeast exonuclease 1.Nucleic acids research · 1998Article
- DNA synthesis on discontinuous templates by human DNA polymerases: implications for non-homologous DNA recombination.Nucleic acids research · 1998Article
- Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammalian cells, the predominant pathway of chromosomal integration of exogenous DNA is random or illegitimate recombination; integration by homologous recombination is infrequent. Homologous recombination is initiated at double-strand DNA breaks which have been acted on by single-strand exonuclease. To further characterize the relationship between illegitimate and homologous recombination, we have investigated whether illegitimate recombination is also preceded by exonuclease digestion. Heteroduplex DNAs which included strand-specific restriction markers at each of four positions were generated. These DNAs were introduced into mouse embryonic stem cells, and stably transformed clones were isolated and analyzed to determine whether there was any strand bias in the retention of restriction markers with respect to their positions. Some of the mismatches appear to have been resolved by mismatch repair. Very significant strand bias was observed in the retention of restriction markers, and there was polarity of marker retention between adjacent positions. We conclude that DNA is frequently subjected to 5'-->3' exonuclease digestion prior to integration by illegitimate recombination and that the length of DNA removed by exonuclease digestion can be extensive. We also provide evidence which suggests that frequent but less extensive 3'-->5' exonuclease processing also occurs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.