ArticleMolecular and cellular biology1997
Comparison of the transactivation domains of Stat5 and Stat6 in lymphoid cells and mammary epithelial cells.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 126 citations in OpenAlex.
- Characterization of fish-specific IFNγ-related binding with a unique receptor complex and signaling through a novel pathway.FEBS open bio · 2024Article
- Establishing a novel and sensitive assay for bioactivity determination of anti-CD25 antibodies.Heliyon · 2023Article
- STAT family of transcription factors in breast cancer: Pathogenesis and therapeutic opportunities and challenges.Seminars in cancer biology · 2022Review
- IL-4 and IL-13 Promote Proliferation of Mammary Epithelial Cells through STAT6 and IRS-1.International journal of molecular sciences · 2021Article
- Redox Regulation of STAT1 and STAT3 Signaling.International journal of molecular sciences · 2020Review
- Analysis of human breast milk cells: gene expression profiles during pregnancy, lactation, involution, and mastitic infection.Functional & integrative genomics · 2016Article
- The spectrum of STAT functions in mammary gland development.JAK-STAT · 2012Review
- Transcriptional regulation by STAT6.Immunologic research · 2011Review
- Stat5a serine 725 and 779 phosphorylation is a prerequisite for hematopoietic transformation.Blood · 2010Article
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- Selective potentiation of Stat-dependent gene expression by collaborator of Stat6 (CoaSt6), a transcriptional cofactor.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Characterization of RNA helicase A as component of STAT6-dependent enhanceosome.Nucleic acids research · 2006Article
- The DNA replication factor MCM5 is essential for Stat1-mediated transcriptional activation.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Identification of p100 as a coactivator for STAT6 that bridges STAT6 with RNA polymerase II.The EMBO journal · 2002Article
- Requirement of Ca2+ and CaMKII for Stat1 Ser-727 phosphorylation in response to IFN-gamma.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Induction of the IL-13 receptor alpha2-chain by IL-4 and IL-13 in human keratinocytes: involvement of STAT6, ERK and p38 MAPK pathways.Oncogene · 2001Article
- Expression level-dependent contribution of glucocorticoid receptor domains for functional interaction with STAT5.Molecular and cellular biology · 2001Article
- Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Antiviral response in cells containing Stat1 with heterologous transactivation domains.Journal of virology · 2001Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stat (signal transducers and activators of transcription) and Jak (Janus kinases) proteins are central components in the signal transduction events in hematopoietic and epithelial cells. They are rapidly activated by various cytokines, hormones, and growth factors. Upon ligand binding and cytokine receptor dimerization, Stat proteins are phosphorylated on tyrosine residues by Jak kinases. Activated Stat proteins form homo- or heterodimers, translocate to the nucleus, and induce transcription from responsive genes. Stat5 and Stat6 are transcription factors active in mammary epithelial cells and immune cells. Prolactin activates Stat5, and interleukin-4 (IL-4) activates Stat6. Both cytokines are able to stimulate cell proliferation, differentiation, and survival. We investigated the transactivation potential of Stat6 and found that it is not restricted to lymphocytes. IL-4-dependent activation of Stat6 was also observed in HC11 mammary epithelial cells. In these cells, Stat6 activation led to the induction of the beta-casein gene promoter. The induction of this promoter was confirmed in COS7 cells. The glucocorticoid receptor was able to further enhance IL-4-induced gene transcription through the action of Stat6. Deletion analysis of the carboxyl-terminal region of Stat6 and recombination of this region with a heterologous DNA binding domain allowed the delimitation and characterization of the transactivation domain of Stat6. The potencies of the transactivation domains of Stat5, Stat6, and viral protein VP16 were compared. Stat6 had a transactivation domain which was about 10-fold stronger than that of Stat5. In pre-B cells (Ba/F3), the transactivation domain of Stat6 was IL-4 regulated, independently from its DNA binding function.
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