ArticleMolecular and cellular biology1997
Analysis of a meiosis-specific URS1 site: sequence requirements and involvement of replication protein A.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Crossover formation and coordinated assembly of synaptonemal complex relies on a direct interaction between Zip1 and Zip3.Genetics · 2026Article
- Crossover formation and coordinated assembly of synaptonemal complex relies on a direct interaction between Zip1 and Zip3.bioRxiv : the preprint server for biology · 2025Article
- Ras and Gpa2 mediate one branch of a redundant glucose signaling pathway in yeast.PLoS biology · 2004Article
- Sum1 and Ndt80 proteins compete for binding to middle sporulation element sequences that control meiotic gene expression.Molecular and cellular biology · 2003Article
- Integrating regulatory motif discovery and genome-wide expression analysis.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Rfm1, a novel tethering factor required to recruit the Hst1 histone deacetylase for repression of middle sporulation genes.Molecular and cellular biology · 2003Article
- The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene.Molecular and cellular biology · 2003Article
- Saccharomyces cerevisiae Mer3 is a DNA helicase involved in meiotic crossing over.Molecular and cellular biology · 2002Article
- Correlating overrepresented upstream motifs to gene expression: a computational approach to regulatory element discovery in eukaryotes.BMC bioinformatics · 2002Article
- RBT1, a novel transcriptional co-activator, binds the second subunit of replication protein A.Nucleic acids research · 2000Article
- Transcriptional regulation of the SMK1 mitogen-activated protein kinase gene during meiotic development in Saccharomyces cerevisiae.Molecular and cellular biology · 1998Article
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Authors and funding
5 authors at 1 institution in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
URS1 is a transcriptional repressor site found in the promoters of a wide variety of yeast genes that are induced under stress conditions. In the context of meiotic promoters, URS1 sites act as repressor sequences during mitosis and function as activator sites during meiosis. We have investigated the sequence requirements of the URS1 site of the meiosis-specific HOP1 gene (URS1H) and have found differences compared with a URS1 site from a nonmeiotic gene. We have also observed that the sequence specificity for meiotic activation at this site differs from that for mitotic repression. Base pairs flanking the conserved core sequence enhance meiotic induction but are not required for mitotic repression of HOP1. Electrophoretic mobility shift assays of mitotic and meiotic cell extracts show a complex pattern of DNA-protein complexes, suggesting that several different protein factors bind specifically to the site. We have determined that one of the complexes of URS1H is formed by replication protein A (RPA). Although RPA binds to the double-stranded URS1H site in vitro, it has much higher affinity for single-stranded than for double-stranded URS1H, and one-hybrid assays suggest that RPA does not bind to this site at detectable levels in vivo. In addition, conditional-lethal mutations in RPA were found to have no effect on URS1H-mediated repression. These results suggest that although RPA binds to URS1H in vitro, it does not appear to have a functional role in transcriptional repression through this site in vivo.
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