ArticleThe Journal of cell biology1997
Chromatin dynamics in interphase nuclei and its implications for nuclear structure.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
79 citing papers in PubMed, 192 citations in OpenAlex.
- The material properties of mitotic chromosomes.Current opinion in structural biology · 2023Review
- In diverse conditions, intrinsic chromatin condensates have liquid-like material properties.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Condensed but liquid-like domain organization of active chromatin regions in living human cells.Science advances · 2023Article
- Visualizing Chromosome Territories and Nuclear Architecture of Large Plant Genomes Using Alien Introgressions.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Mammalian DNA Replication Timing.Cold Spring Harbor perspectives in biology · 2021Review
- Cell cycle dynamics of lamina-associated DNA.EMBO reports · 2020Article
- The rich inner life of the cell nucleus: dynamic organization, active flows, and emergent rheology.Biophysical reviews · 2020Review
- Monitoring the spatio-temporal organization and dynamics of the genome.Nucleic acids research · 2020Review
- The self-stirred genome: large-scale chromatin dynamics, its biophysical origins and implications.Current opinion in genetics & development · 2020Review
- Control of DNA replication timing in the 3D genome.Nature reviews. Molecular cell biology · 2019Review
- Nuclear Disposition of Alien Chromosome Introgressions into Wheat and Rye Using 3D-FISH.International journal of molecular sciences · 2019Article
- Report of the AAPM TG-256 on the relative biological effectiveness of proton beams in radiation therapy.Medical physics · 2019Article
- A new application of the phase-field method for understanding the mechanisms of nuclear architecture reorganization.Journal of mathematical biology · 2017Article
- High resolution imaging reveals heterogeneity in chromatin states between cells that is not inherited through cell division.BMC cell biology · 2016Article
- Chromosomes at Work: Organization of Chromosome Territories in the Interphase Nucleus.Journal of cellular biochemistry · 2016Article
- Uniform Contraction-Expansion Description of Relative Centromere and Telomere Motion.Biophysical journal · 2015Article
- Cytoskeletal tension induces the polarized architecture of the nucleus.Biomaterials · 2015Article
- Cell type specific alterations in interchromosomal networks across the cell cycle.PLoS computational biology · 2014Article
- Nuclear architecture as an epigenetic regulator of neural development and function.Neuroscience · 2014Review
- DNA in motion during double-strand break repair.Trends in cell biology · 2013Review
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Translational dynamics of chromatin in interphase nuclei of living Swiss 3T3 and HeLa cells was studied using fluorescence microscopy and fluorescence recovery after photobleaching. Chromatin was fluorescently labeled using dihydroethidium, a membrane-permeant derivative of ethidium bromide. After labeling, a laser was used to bleach small (approximately 0.4 microm radius) spots in the heterochromatin and euchromatin of cells of both types. These spots were observed to persist for >1 h, implying that interphase chromatin is immobile over distance scales >/=0.4 microm. Over very short times (<1 s), a partial fluorescence recovery within the spots was observed. This partial recovery is attributed to independent dye motion, based on comparison with results obtained using ethidium homodimer-1, which binds essentially irreversibly to nucleic acids. The immobility observed here is consistent with chromosome confinement to domains in interphase nuclei. This immobility may reflect motion-impeding steric interactions that arise in the highly concentrated nuclear milieu or outright attachment of the chromatin to underlying nuclear substructures, such as nucleoli, the nuclear lamina, or the nuclear matrix.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.