ArticleIn vitro cellular & developmental biology. Animal1997
Three-dimensional culture of a mixed mullerian tumor of the ovary: expression of in vivo characteristics.
Article in In vitro cellular & developmental biology. Animal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 50 citations in OpenAlex.
- Extraterrestrial Gynecology: Could Spaceflight Increase the Risk of Developing Cancer in Female Astronauts? An Updated Review.International journal of molecular sciences · 2022Review
- Three-Dimensional Modelling of Ovarian Cancer: From Cell Lines to Organoids for Discovery and Personalized Medicine.Frontiers in bioengineering and biotechnology · 2022Review
- PTEN/FOXO3/AKT pathway regulates cell death and mediates morphogenetic differentiation of Colorectal Cancer Cells under Simulated Microgravity.Scientific reports · 2017Article
- 3D tissue-like assemblies: A novel approach to investigate virus-cell interactions.Methods (San Diego, Calif.) · 2015Article
- Using space-based investigations to inform cancer research on Earth.Nature reviews. Cancer · 2013Review
- Differentiation of human mesenchymal stem cell spheroids under microgravity conditions.Cell regeneration (London, England) · 2012Article
- An update to space biomedical research: tissue engineering in microgravity bioreactors.BioImpacts : BI · 2012Article
- Generation of a tumor spheroid in a microgravity environment as a 3D model of melanoma.In vitro cellular & developmental biology. Animal · 2009Article
- Simulated microgravity impairs respiratory burst activity in human promyelocytic cells.In vitro cellular & developmental biology. Animal · 2001Article
- Fetal thymic organ culture in rotating bioreactors.In vitro cellular & developmental biology. Animal · 1999Review
- Cytogenetic, morphologic and oncogene analysis of a cell line derived from a heterologous mixed mullerian tumor of the ovary.In vitro cellular & developmental biology. Animal · 1997Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Rotating-Wall Vessel (RWV) is a novel in vitro cell culture system used to successfully culture a cell line derived from a heterologous mixed mullerian tumor cell of the ovary. Although the original tumor was comprised of both epithelial and mesodermal components, long-term culture in conventional flasks established a cell line from this tumor with homogeneous epitheliallike growth characteristics (1). Cells from Passage 36 were seeded into a Rotating-Wall Vessel containing Cytodex-3 microcarrier beads. Scanning electron micrographs of tumor cells cultured for 32 d in the RWV showed the presence of heterogeneous cell populations organized into three-dimensional tissuelike architecture. Immunocytochemical analysis confirmed the cellular heterogeneity, as demonstrated by expression of both epithelial and mesenchymal antigens. Reverse transcription polymerase chain reaction amplification demonstrated the presence of mRNA for cellular oncogenes HER-2/neu, H-ras, K-ras, and tumor suppressor p53. Thus, there are two advantages to propagation of tissue in the RWV culture system:(a) tissue diversification representing populations present in the original tumor, and (b) the three-dimensional freedom to organize tissues morphologically akin to those observed in vivo. These data indicate that the RWV culture system is suitable for generating large quantities of ovarian tumor cells in vitro that are amenable to immunocytochemical, oncogenic, morphologic characteristics demonstrated in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.