ArticleJournal of clinical microbiology1997
Evidence for numerous omp1 alleles of porcine Chlamydia trachomatis and novel chlamydial species obtained by PCR.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 83 citations in OpenAlex.
- Reliable and Sensitive Nested PCR for the Detection ofMicroorganisms · 2021Article
- Detection and identification ofIranian journal of microbiology · 2020Article
- Establishment of a Multiplex Real-Time TaqMan-MGB Polymerase Chain Reaction (PCR) Method for the Simultaneous Detection of Three Animal Chlamydia Species.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- First report of caprine abortions due to Chlamydia abortus in Argentina.Veterinary medicine and science · 2019Article
- Detection of Chlamydia infection in Peromyscus species rodents from sylvatic and laboratory sources.Pathogens and disease · 2016Article
- Genetic and antigenic analysis of Chlamydia pecorum strains isolated from calves with diarrhea.The Journal of veterinary medical science · 2015Article
- Defining species-specific immunodominant B cell epitopes for molecular serology of Chlamydia species.Clinical and vaccine immunology : CVI · 2015Article
- Isolation and molecular identification of Mycoplasma equigenitalium from equine genital tracts in northern India.Iranian journal of veterinary research · 2015Article
- Molecular Detection and Genotyping of Chlamydia psittaci in Captive Psittacines from Costa Rica.Veterinary medicine international · 2013Article
- Chlamydia psittaci in birds of prey, Sweden.Infection ecology & epidemiology · 2012Article
- Asymptomatic endemic Chlamydia pecorum infections reduce growth rates in calves by up to 48 percent.PloS one · 2012Article
- Investigation of Chlamydophila spp. in dairy cows with reproductive disorders.Acta veterinaria Scandinavica · 2008Article
- Chlamydophila spp. infection in horses with recurrent airway obstruction: similarities to human chronic obstructive disease.Respiratory research · 2008Article
- Therapeutic Chlamydophila abortus and C. pecorum vaccination transiently reduces bovine mastitis associated with Chlamydophila infection.Infection and immunity · 2007Article
- Evidence of infection in tortoises by Chlamydia-like organisms that are genetically distinct from known Chlamydiaceae species.Veterinary research communications · 2005Article
- High prevalence of chlamydial (Chlamydophila psittaci) infection in fetal membranes of aborted equine fetuses.Veterinary research communications · 2005Article
- Bovine Chlamydophila spp. infection: do we underestimate the impact on fertility?Veterinary research communications · 2005Review
- Evaluation of lung function in pigs either experimentally or naturally infected with Chlamydiaceae.Veterinary research communications · 2005Article
- A comparative ultrastructural and molecular biological study on Chlamydia psittaci infection in alpha-1 antitrypsin deficiency and non-alpha-1 antitrypsin deficiency emphysema versus lung tissue of patients with hamartochondroma.BMC infectious diseases · 2004Article
- Specificity and performance of PCR detection assays for microbial pathogens.Molecular biotechnology · 2004Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A nested PCR for genus-specific amplification of the Chlamydia omp1 locus was established. This PCR detected single template molecules in 200-microl specimen aliquots. Amplified chlamydial omp1 alleles were typed by heminested species PCRs and allele PCRs. We applied this method to 407 specimens from several host animals with various clinical conditions, and we detected prevalences of chlamydiae from 6 to 50%. Amplicons from peacock enteritis and equine infertility specimens were not typeable according to present omp1 allelic criteria for the chlamydial species. DNA sequencing revealed novel omp1 alleles which were 29.9 and 47.6% divergent in the deduced peptide sequences from the most closely related chlamydiae. Phylogenetic reconstruction indicated segregation of these alleles from the current four chlamydial species (90 and 97% bootstrap support), thus strongly suggesting the existence of additional chlamydial species. Allele typing of amplicons from swine with intestinal, urogenital, and respiratory infections demonstrated several unique omp1 allelic variants of Chlamydia trachomatis. These novel alleles had deduced peptide sequences which were 11.6 to 19% divergent from porcine C. trachomatis S45. Mutations were clustered in the C-terminal region of variable segment IV of the omp1 locus encoding subspecies and serovar determinants of the chlamydial major outer membrane protein, thus implying that there are numerous serovars of porcine C. trachomatis. These results demonstrate the need for routine application of sensitive genus-specific detection of chlamydiae in animal specimens and suggest a more prominent role than anticipated for chlamydiae in animal diseases.
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