ArticleProtein science : a publication of the Protein Society1997
Hydrogen exchange properties of proteins in native and denatured states monitored by mass spectrometry and NMR.
Article in Protein science : a publication of the Protein Society, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 98 citations in OpenAlex.
- On-tissue Direct Monitoring of Global Hydrogen/Deuterium Exchange by MALDI Mass Spectrometry: Tissue Deuterium Exchange Mass Spectrometry (TDXMS).Molecular & cellular proteomics : MCP · 2016Article
- Estimation of Hydrogen-Exchange Protection Factors from MD Simulation Based on Amide Hydrogen Bonding Analysis.Journal of chemical information and modeling · 2015Article
- Terahertz optical measurements of correlated motions with possible allosteric function.Biophysical reviews · 2015Review
- Article
- Two amyloid States of the prion protein display significantly different folding patterns.Journal of molecular biology · 2010Article
- Double superhelix model of high density lipoprotein.The Journal of biological chemistry · 2009Article
- The gramicidin dimer shows both EX1 and EX2 mechanisms of H/D exchange.Journal of the American Society for Mass Spectrometry · 2009Article
- Scope and utility of hydrogen exchange as a tool for mapping landscapes.Protein science : a publication of the Protein Society · 2007Article
- Identification and characterization of EX1 kinetics in H/D exchange mass spectrometry by peak width analysis.Journal of the American Society for Mass Spectrometry · 2006Article
- Transfer of structural elements from compact to extended states in unsolvated ubiquitin.Journal of the American Chemical Society · 2006Article
- Dynamic motions of free and bound O29 scaffolding protein identified by hydrogen deuterium exchange mass spectrometry.Protein science : a publication of the Protein Society · 2006Article
- Destabilization of apoprotein is insufficient to explain Cu,Zn-superoxide dismutase-linked ALS pathogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Structural features of interferon-gamma aggregation revealed by hydrogen exchange.Protein science : a publication of the Protein Society · 2002Article
- Automatic analysis of hydrogen/deuterium exchange mass spectra of peptides and proteins using calculations of isotopic distributions.Journal of the American Society for Mass Spectrometry · 2001Article
- An electrospray ionization mass spectrometry investigation of 1-anilino-8-naphthalene-sulfonate (ANS) binding to proteins.Journal of the American Society for Mass Spectrometry · 2001Article
- Inter- and intra-molecular migration of peptide amide hydrogens during electrospray ionization.Journal of the American Society for Mass Spectrometry · 2001Article
- The methanol-induced conformational transitions of beta-lactoglobulin, cytochrome c, and ubiquitin at low pH: a study by electrospray ionization mass spectrometry.Journal of the American Society for Mass Spectrometry · 2001Article
- Folding of a three-stranded coiled coil.Protein science : a publication of the Protein Society · 2000Article
- Solvent effects on the conformation of the transmembrane peptide gramicidin A: insights from electrospray ionization mass spectrometry.Biophysical journal · 2000Article
- Matrix-assisted laser desorption ionization hydrogen/deuterium exchange studies to probe peptide conformational changes.Journal of the American Society for Mass Spectrometry · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The extent of deuterium labeling of hen lysozyme, its three-disulfide derivative, and the homologous alpha-lactalbumins, has been measured by both mass spectrometry and NMR. Different conformational states of the proteins were produced by varying the solution conditions. Alternate protein conformers were found to contain different numbers of 2H atoms. Furthermore, measurement in the gas phase of the mass spectrometer or directly in solution by NMR gave consistent results. The unique ability of mass spectrometry to distinguish distributions of 2H atoms in protein molecules is exemplified using samples prepared to contain different populations of 2H-labeled protein. A comparison of the peak widths of bovine alpha-lactalbumin in alternate solution conformations but containing the same average number of 2H atoms showed dramatic differences due to different 2H distributions in the two protein conformers. Measurement of 2H distributions by ESI-MS enabled characterization of conformational averaging and structural heterogeneity. In addition, a time course for hydrogen exchange was examined and the variation in distributions of 2H atom compared with simulations for different hydrogen exchange models. The results clearly show that exchange from the native state of bovine alpha-lactalbumin at 15 degrees C is dominated by local unfolding events.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.