ArticleProceedings of the National Academy of Sciences of the United States of America1997
Continuous in vitro propagation of the malaria parasite Plasmodium vivax.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Evaluation of Reproducibility of a Sporozoite Challenge Model for Plasmodium Vivax in Human Volunteers
Establishment of a Sporozoite Challenge Model for Plasmodium Vivax in Human Volunteers
Who cites it
59 citing papers in PubMed, 139 citations in OpenAlex.
- Beyond the mouse: organoids, spheroids, and organs-on-chips as the (inevitable) future of malaria research?Malaria journal · 2026Review
- A transfer learning approach to identify Plasmodium in microscopic images.PLoS computational biology · 2024Article
- Development of a Plasmodium vivax biobank for functional ex vivo assays.Malaria journal · 2023Article
- Culture and transfection: Two major bottlenecks in understandingFrontiers in microbiology · 2023Review
- The Black Box of Cellular and Molecular Events ofInternational journal of molecular sciences · 2022Review
- The challenges ofFrontiers in immunology · 2022Article
- Using Plasmodium knowlesi as a model for screening Plasmodium vivax blood-stage malaria vaccine targets reveals new candidates.PLoS pathogens · 2021Article
- Alternative Invasion Mechanisms and Host Immune Response toMicroorganisms · 2020Review
- On the survival of 48 h Plasmodium vivax Aotus monkey-derived ex vivo cultures: the role of leucocytes filtration and chemically defined lipid concentrate media supplementation.Malaria journal · 2020Article
- A Way Forward for Culturing Plasmodium vivax.Trends in parasitology · 2020Review
- From marginal to essential: the golden thread between nutrient sensing, medium composition and Plasmodium vivax maturation in in vitro culture.Malaria journal · 2019Review
- Functional genomics of simian malaria parasites and host-parasite interactions.Briefings in functional genomics · 2019Review
- Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes.Scientific reports · 2019Article
- Divergent roles for the RH5 complex components, CyRPA and RIPR in human-infective malaria parasites.PLoS pathogens · 2019Article
- Plasmodium vivax in vitro continuous culture: the spoke in the wheel.Malaria journal · 2018Review
- Malaria in India: The Need for New Targets for Diagnosis and Detection of Plasmodium vivax.Proteomics. Clinical applications · 2018Review
- Article
- Enhanced Ex Vivo Plasmodium vivax Intraerythrocytic Enrichment and Maturation for Rapid and Sensitive Parasite Growth Assays.Antimicrobial agents and chemotherapy · 2018Article
- Advancing Research Models and Technologies to Overcome Biological Barriers to Plasmodium vivax Control.Trends in parasitology · 2018Review
- Molecular interactions governing host-specificity of blood stage malaria parasites.Current opinion in microbiology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The difficulty in controlling Plasmodium vivax, the most common cause of human malaria, has been complicated by growing drug resistance. We have established a method to cycle parasite generations in continuous culture using human blood cells. Chesson strain parasites were passaged from owl monkey erythrocytes to human reticulocytes in McCoy's 5A medium modified with L-glutamine with 25 mM Hepes buffer supplemented with 20% AB+ human serum. Reticulocytes were separated by differential centrifugation in homologous plasma from the peripheral blood of a hemochromatosis patient. Parasites were grown during each 48-hr cycle in a static candle jar environment until the beginning of schizogony, at about 36-40 hr, when reticulocytes were added and cultures transferred to a shaker for 10-12 hr. The addition of a concentration of 10% reticulocytes resulted in stabilizing parasite densities between 0.28 and 0.57 after cycle 3 and increasing the total number of parasites at least 2-fold with each generational cycle. Cultured parasites successfully infected an owl monkey. The morphology of cultured parasites was typical of P. vivax, with highly ameboid trophozoites evident; however, infected erythrocytes were enlarged and distorted on thin film preparations. The species identity of cultivated parasites was confirmed by analysis of the A and C 18S rRNA genes from genomic DNA and expression of only the A gene during erythrocytic asexual growth. The ability to culture P. vivax opens new opportunities to develop vaccines, test drugs, and clone parasites for genome sequencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.