ArticleProceedings of the National Academy of Sciences of the United States of America1997
In vivo repopulating hematopoietic stem cells are present in the murine yolk sac at day 9.0 postcoitus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 245 citations in OpenAlex.
- Balancing hematopoietic stem cell self-renewal and differentiation activities throughout ontogeny and aging.Experimental hematology · 2025Review
- Unraveling hematopoietic stem cell ontogeny through single-cell multi-omics approaches.Frontiers in cell and developmental biology · 2025Review
- Layered immunity and layered leukemogenicity: Developmentally restricted mechanisms of pediatric leukemia initiation.Immunological reviews · 2023Review
- Specification of hematopoietic stem cells in mammalian embryos: a rare or frequent event?Blood · 2022Article
- Review
- Engineering a niche supporting hematopoietic stem cell development using integrated single-cell transcriptomics.Nature communications · 2022Article
- Review
- Adult-repopulating lymphoid potential of yolk sac blood vessels is not confined to arterial endothelial cells.Science China. Life sciences · 2021Article
- Transcriptional reprogramming in neonatal hematopoietic stem and progenitor cells.Experimental hematology · 2021Review
- The hemogenic endothelium: a critical source for the generation of PSC-derived hematopoietic stem and progenitor cells.Cellular and molecular life sciences : CMLS · 2021Review
- Two macrophages, osteoclasts and microglia: from development to pleiotropy.Bone research · 2021Review
- Absence of CD11a Expression Identifies Embryonic Hematopoietic Stem Cell PrecursorsFrontiers in cell and developmental biology · 2021Article
- Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells.Cell reports · 2020Article
- Transcriptome Dynamics of Hematopoietic Stem Cell Formation Revealed Using a Combinatorial Runx1 and Ly6a Reporter System.Stem cell reports · 2020Article
- Clones assemble! The clonal complexity of blood during ontogeny and disease.Experimental hematology · 2020Review
- Chasing Mavericks: The quest for defining developmental waves of hematopoiesis.Current topics in developmental biology · 2019Review
- Murine hematopoietic stem cell activity is derived from pre-circulation embryos but not yolk sacs.Nature communications · 2018Article
- Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture.Journal of visualized experiments : JoVE · 2018Article
- Many layers of embryonic hematopoiesis: new insights into B-cell ontogeny and the origin of hematopoietic stem cells.Experimental hematology · 2018Review
- Lifelong haematopoiesis is established by hundreds of precursors throughout mammalian ontogeny.Nature cell biology · 2017Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The murine yolk sac, being the first site of embryonic blood cell production, has long been theorized to contain the migrating hematopoietic stem cells (HSC) that seed the liver and initiate hematopoiesis on day 10.0 postcoitus (pc). However, it remains controversial whether yolk sac cells isolated before day 11.0 pc possess any long-term repopulating HSC activity upon transplantation into adult recipient mice. We hypothesized that failure to demonstrate engraftment of day <11.0 yolk sac cells in adult hosts may result from an inability of yolk sac cells to home to the active adult hematopoietic sites (spleen and bone marrow). In the present studies, we transplanted yolk sac cells into conditioned newborn mice in whom the liver, as well as the spleen and bone marrow, concomitantly function as a site of blood cell formation. We report that yolk sac cells isolated from day 9.0 pc embryos provide long-term multilineage reconstitution for at least 11 months in primary conditioned newborn mice and for at least 6 months in secondary recipients. Donor yolk sac HSC progeny repopulated mature peripheral blood, thymus, spleen, and bone marrow lymphoid, myeloid, and erythroid compartments. Thus, day 9.0 pc yolk sac HSC can contribute to definitive multilineage hematopoiesis in transplanted recipients. Determination of HSC activity in the day 9.0 pc murine yolk sac suggests that yolk sac HSC are available to seed the liver on day 10.0 pc when definitive hematopoiesis is initiated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.