ArticleProceedings of the National Academy of Sciences of the United States of America1997
Pacifastin, a novel 155-kDa heterodimeric proteinase inhibitor containing a unique transferrin chain.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 140 citations in OpenAlex.
- Structure-guided analysis of divergent homologs unveils deep ancestry and arthropod specialization of the pacifastin family.Scientific reports · 2026Article
- An insight into the salivary gland content of the human body louse, Pediculus humanus.Scientific reports · 2025Article
- The hematopoietic tissue of the freshwater crayfish, Pacifastacus leniusculus: organization and expression analysis.Cell and tissue research · 2025Article
- Host-pathogen coevolution drives innate immune response to Aphanomyces astaci infection in freshwater crayfish: transcriptomic evidence.BMC genomics · 2022Article
- Characterization of hemocytes and hematopoietic cells of a freshwater crayfish based on single-cell transcriptome analysis.iScience · 2022Article
- Changes in composition and levels of hemolymph proteins during metamorphosis of Manduca sexta.Insect biochemistry and molecular biology · 2020Article
- Pulse magnetization elicits differential gene expression in the central nervous system of the Caribbean spiny lobster, Panulirus argus.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2020Article
- Article
- Transcriptional Identification of Related Proteins in the Immune System of the CrayfishHigh-throughput · 2018Article
- Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors.BMC genomics · 2018Article
- Screening, large-scale production and structure-based classification of cystine-dense peptides.Nature structural & molecular biology · 2018Article
- Exploring the molecular complexity of Triatoma dimidiata sialome.Journal of proteomics · 2018Article
- A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease.PLoS neglected tropical diseases · 2016Article
- The trypsin inhibitor panulirin regulates the prophenoloxidase-activating system in the spiny lobster Panulirus argus.The Journal of biological chemistry · 2013Article
- A double WAP domain-containing protein Es-DWD1 from Eriocheir sinensis exhibits antimicrobial and proteinase inhibitory activities.PloS one · 2013Article
- Article
- Identification, distribution and molecular evolution of the pacifastin gene family in Metazoa.BMC evolutionary biology · 2009Article
- A novel locust (Schistocerca gregaria) serine protease inhibitor with a high affinity for neutrophil elastase.The Biochemical journal · 2006Article
- Annotation of novel neuropeptide precursors in the migratory locust based on transcript screening of a public EST database and mass spectrometry.BMC genomics · 2006Article
- Evidence for proteins involved in prophenoloxidase cascade Eisenia fetida earthworms.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 155-kDa proteinase inhibitor, pacifastin, from plasma of the freshwater crayfish, Pacifastacus leniusculus, was found to be composed of two covalently linked subunits. The two subunits are encoded by two different mRNAs, which were cloned and sequenced. The heavy chain of pacifastin (105 kDa) is related to transferrins, containing three transferrin lobes, two of which seem to be active for iron binding. The light chain of pacifastin (44 kDa) is the inhibitory subunit, and has nine cysteine-rich inhibitory domains that are homologous to each other and to low molecular weight proteinase inhibitors isolated from the grasshopper, Locusta migratoria. The nine light chain domains and the Locusta inhibitors share a characteristic cysteine array (Cys-Xaa9-12-Cys-Xaa2-Cys-Xaa-Cys-Xaa6-8-Cys-Xaa4++ +-Cys) distinct from any described proteinase inhibitor family, suggesting that they constitute a new family of proteinase inhibitors. Pacifastin is the first known protein that has combined properties of a transferrin-like molecule and a proteinase inhibitor.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.