ArticleMolecular biology of the cell1997
Dual role of the actin cytoskeleton in regulating cell adhesion mediated by the integrin lymphocyte function-associated molecule-1.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 162 citations in OpenAlex.
- LRBA regulates actin cytoskeleton dynamics through NMIIA during B cell immune responses.EMBO reports · 2026Article
- Temporal and Spatial Dynamics of Astroglial Reaction and Immune Response in Cuprizone-Induced Demyelination.Neurotoxicity research · 2020Article
- Actin Cytoskeleton Straddling the Immunological Synapse between Cytotoxic Lymphocytes and Cancer Cells.Cells · 2019Review
- T-Cell Mechanobiology: Force Sensation, Potentiation, and Translation.Frontiers in physics · 2019Article
- Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- αII-spectrin in T cells is involved in the regulation of cell-cell contact leading to immunological synapse formation?PloS one · 2017Article
- Importance of Interaction between Integrin and Actin Cytoskeleton in Suspension Adaptation of CHO cells.Applied biochemistry and biotechnology · 2016Article
- TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse.The Journal of cell biology · 2015Article
- Defective actin accumulation impairs human natural killer cell function in patients with dedicator of cytokinesis 8 deficiency.The Journal of allergy and clinical immunology · 2013Article
- Biophysical description of multiple events contributing blood leukocyte arrest on endothelium.Frontiers in immunology · 2013Article
- Lateral mobility of individual integrin nanoclusters orchestrates the onset for leukocyte adhesion.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Priming of eosinophils by GM-CSF is mediated by protein kinase CbetaII-phosphorylated L-plastin.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Phosphorylation of paxillin at threonine 538 by PKCdelta regulates LFA1-mediated adhesion of lymphoid cells.Journal of cell science · 2010Article
- The multifaceted profile of activated microglia.Molecular neurobiology · 2009Review
- Pivotal advance: CEACAM1 is a negative coreceptor for the B cell receptor and promotes CD19-mediated adhesion of B cells in a PI3K-dependent manner.Journal of leukocyte biology · 2009Article
- Transmigration of neutrophils across inflamed endothelium is signaled through LFA-1 and Src family kinase.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Rho-ROCK signaling differentially regulates chondrocyte spreading on fibronectin and bone sialoprotein.American journal of physiology. Cell physiology · 2008Article
- Alterations in blood-brain barrier ICAM-1 expression and brain microglial activation after lambda-carrageenan-induced inflammatory pain.American journal of physiology. Heart and circulatory physiology · 2006Article
- Actin depolymerization transduces the strength of B-cell receptor stimulation.Molecular biology of the cell · 2005Article
- SRC-dependent outside-in signalling is a key step in the process of autoregulation of beta2 integrins in polymorphonuclear cells.The Biochemical journal · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular signals are required to activate the leukocyte-specific adhesion receptor lymphocyte function-associated molecule-1 (LFA-1; CD11a/CD18) to bind its ligand, intracellular adhesion molecule-1 (ICAM-1). In this study, we investigated the role of the cytoskeleton in LFA-1 activation and demonstrate that filamentous actin (F-actin) can both enhance and inhibit LFA-1-mediated adhesion, depending on the distribution of LFA-1 on the cell surface. We observed that LFA-1 is already clustered on the cell surface of interleukin-2/phytohemagglutinin-activated lymphocytes. These cells bind strongly ICAM-1 and disruption of the actin cytoskeleton inhibits adhesion. In contrast to interleukin-2/phytohemagglutinin-activated peripheral blood lymphocytes, resting lymphocytes, which display a homogenous cell surface distribution of LFA-1, respond poorly to intracellular signals to bind ICAM-1, unless the actin cytoskeleton is disrupted. On resting peripheral blood lymphocytes, uncoupling of LFA-1 from the actin cytoskeleton induces clustering of LFA-1 and this, along with induction of a high-affinity form of LFA-1, via "inside-out" signaling, results in enhanced binding to ICAM-1, which is dependent on intact intermediate filaments, microtubules, and metabolic energy. We hypothesize that linkage of LFA-1 to cytoskeletal elements prevents movement of LFA-1 over the cell surface, thus inhibiting clustering and strong ligand binding. Release from these cytoskeletal elements allows lateral movement and activation of LFA-1, resulting in ligand binding and "outside-in" signaling, that subsequently stimulates actin polymerization and stabilizes cell adhesion.
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