ArticleMolecular biology of the cell1997
Topogenesis of a nucleolar protein: determination of molecular segments directing nucleolar association.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Identification and fine mapping of nuclear and nucleolar localization signals within the human ribosomal protein S17.PloS one · 2015Article
- An IKKα-nucleophosmin axis utilizes inflammatory signaling to promote genome integrity.Cell reports · 2013Article
- Mislocalization or low expression of mutated Shwachman-Bodian-Diamond syndrome protein.International journal of hematology · 2011Article
- NPM1/B23: A Multifunctional Chaperone in Ribosome Biogenesis and Chromatin Remodeling.Biochemistry research international · 2011Article
- Posttranscriptional regulation of chicken ccn2 gene expression by nucleophosmin/B23 during chondrocyte differentiation.Molecular and cellular biology · 2008Article
- Insights into nuclear organization in plants as revealed by the dynamic distribution of Arabidopsis SR splicing factors.The Plant cell · 2006Article
- Article
- Nucleophosmin and human cancer.Cancer detection and prevention · 2006Review
- NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin.Molecular biology of the cell · 2004Article
- The RNA binding activity of a ribosome biogenesis factor, nucleophosmin/B23, is modulated by phosphorylation with a cell cycle-dependent kinase and by association with its subtype.Molecular biology of the cell · 2002Article
- Conventional and nonconventional roles of the nucleolus.International review of cytology · 2002Review
- Identification of a sequence element directing a protein to nuclear speckles.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Localization in the nucleolus and coiled bodies of protein subunits of the ribonucleoprotein ribonuclease P.The Journal of cell biology · 1999Article
- Nuclear activities of basic fibroblast growth factor: potentiation of low-serum growth mediated by natural or chimeric nuclear localization signals.Molecular biology of the cell · 1999Article
- Molecular characterization of a novel, widespread nuclear protein that colocalizes with spliceosome components.Molecular biology of the cell · 1998Article
- The yeast SEN1 gene is required for the processing of diverse RNA classes.Nucleic acids research · 1997Article
- Identification of a small, very acidic constitutive nucleolar protein (NO29) as a member of the nucleoplasmin family.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Assembly and disassembly of the nucleolus during the cell cycle.Nucleus (Austin, Tex.)Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify the element(s) in nucleolar proteins which determine nucleolus-specific topogenesis, we have used different kinds of cDNA constructs encoding various chimeric combinations of mutants of the constitutive nucleolar protein NO38 (B23): 1) with an amino terminally placed short "myc tag"; 2) with two different carboxyl terminally attached large alpha-helical coiled coil structures, the lamin A rod domain or the rod domain of vimentin; 3) with the sequence-related nucleoplasmic histone-binding protein nucleo-plasmin; and 4) with the soluble cytoplasmic protein pyruvate kinase. To avoid the problem of formation of complexes with endogenous wild-type (wt) molecules and "piggyback" localization, special care was taken to secure that the mutants and chimeras used did not oligomerize as is typical of protein NO38 (B23). Using microinjection and transfection of cultured cells, we found that the segment comprising the amino-terminal 123 amino acids (aa) alone was sufficient to effect nucleolar accumulation of the construct molecules, including the chimeras with the entire rod domains of lamin A and vimentin. However, when the amino-terminal 109 aa were deleted, the molecules still associated with the nucleolus. The results of further deletion experiments and of domain swaps with nucleoplasmin all point to the topogenic importance of two independent molecular regions located at both the amino- and carboxyl-terminal end. Our definition of dominant elements determining the nucleolar localization of protein NO38 (B23) as well as of diverse nonnucleolar proteins will help to identify its local binding partner(s) and functions, the construction of probes examining other proteins or sequence elements within the nucleolar microenvironment, and the generation of cells with an altered nuclear architecture.
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