ArticleJournal of virology1997
Cloning of the rainbow trout (Oncorhynchus mykiss) Mx2 and Mx3 cDNAs and characterization of trout Mx protein expression in salmon cells.
Article in Journal of virology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 172 citations in OpenAlex.
- Production of biologically active recombinant salmon calcitonin in Escherichia coli and fish cell line.Archives of microbiology · 2025Article
- Review
- Developing a Virus-Binding Bacterium Expressing Mx Protein on the Bacterial Surface to Prevent Grouper Nervous Necrosis Virus Infection.Journal of microbiology and biotechnology · 2021Article
- Article
- Genetic and transcriptomic analyses provide new insights on the early antiviral response to VHSV in resistant and susceptible rainbow trout.BMC genomics · 2018Article
- Insights into the antiviral immunity against grass carp (Ctenopharyngodon idella) reovirus (GCRV) in grass carp.Journal of immunology research · 2015Review
- Innate Multigene Family Memories Are Implicated in the Viral-Survivor Zebrafish Phenotype.PloS one · 2015Article
- Immunity to fish rhabdoviruses.Viruses · 2012Review
- Genetic resistance to rhabdovirus infection in teleost fish is paralleled to the derived cell resistance status.PloS one · 2012Article
- Protective roles of grass carp Ctenopharyngodon idella Mx isoforms against grass carp reovirus.PloS one · 2012Article
- Restricted growth of U-type infectious haematopoietic necrosis virus (IHNV) in rainbow trout cells may be linked to casein kinase II activity.Journal of fish diseases · 2011Article
- A nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth.PloS one · 2011Article
- Structural and functional studies of STAT1 from Atlantic salmon (Salmo salar).BMC immunology · 2010Article
- The interplay between infectious pancreatic necrosis virus (IPNV) and the IFN system: IFN signaling is inhibited by IPNV infection.Virus research · 2009Article
- Positive diversifying selection in avian Mx genes.Immunogenetics · 2008Article
- Inhibition of infectious pancreatic necrosis virus replication by atlantic salmon Mx1 protein.Journal of virology · 2004Article
- Differential expression of two Carassius auratus Mx genes in cultured CAB cells induced by grass carp hemorrhage virus and interferon.Immunogenetics · 2004Article
- Molecular cloning of the pufferfish (Takifugu rubripes) Mx gene and functional characterization of its promoter.Immunogenetics · 2003Article
- Survey of transcript expression in rainbow trout leukocytes reveals a major contribution of interferon-responsive genes in the early response to a rhabdovirus infection.Journal of virology · 2002Article
- Infectious hematopoietic necrosis virus matrix protein inhibits host-directed gene expression and induces morphological changes of apoptosis in cell cultures.Journal of virology · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two rainbow trout (Oncorhynchus mykiss) Mx cDNAs were cloned by using RACE (rapid amplification of cDNA ends) PCR and were designated RBTMx2 and RBTMx3. The deduced RBTMx2 and RBTMx3 proteins were 636 and 623 amino acids in length with molecular masses of 72 and 70.8 kDa, respectively. These proteins, along with the previously described RBTMx1 protein (G. D. Trobridge and J. A. Leong, J. Interferon Cytokine Res. 15:691-702, 1995), have between 88.7 and 96.6% identity at the amino acid level. All three proteins contain the tripartite GTP binding domain and leucine zipper motif common to Mx proteins. A monospecific polyclonal antiserum to an Escherichia coli-expressed fragment of RBTMx3 was generated, and that reagent was found to react with all three rainbow trout Mx proteins. Subsequently, endogenous Mx production in RTG-2 cells induced with poly(IC) double-stranded RNA was detected by immunoblot analysis. The cellular localization of the rainbow trout proteins was determined by transient expression of the RBTMx cDNAs in CHSE-214 (chinook salmon embryo) cells. A single-cell transient-transfection assay was used to examine the ability of each Mx cDNA clone to inhibit replication of the fish rhabdovirus infectious hematopoietic necrosis virus (IHNV). No significant inhibition in the accumulation of the IHNV nucleoprotein was observed in cells expressing either trout Mx1, Mx2, or Mx3 in transiently transfected cells.
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