ArticleMolecular biology of the cell1997
Endocytic trafficking of megalin/RAP complexes: dissociation of the complexes in late endosomes.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 99 citations in OpenAlex.
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- Optimizing renal transporter immunodetection: consequences of freeze-thaw during sample preparation.American journal of physiology. Renal physiology · 2024Article
- Cryo-EM structures elucidate the multiligand receptor nature of megalin.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Receptor-associated protein impairs ligand binding to megalin and megalin-dependent endocytic flux in proximal tubule cells.American journal of physiology. Renal physiology · 2023Article
- Article
- Assessment of endocytic traffic and Ocrl function in the developing zebrafish neuroepithelium.Journal of cell science · 2022Article
- Renal Handling of Albumin-From Early Findings to Current Concepts.International journal of molecular sciences · 2021Review
- Kinetic analysis of transcellular passage of the cobalamin-transcobalamin complex in Caco-2 monolayers.Molecular biology of the cell · 2019Article
- Desmoglein 3 regulates membrane trafficking of cadherins, an implication in cell-cell adhesion.Cell adhesion & migration · 2017Article
- Receptor-Mediated Endocytosis in the Proximal Tubule.Annual review of physiology · 2017Review
- Recombinant Expression of the Full-length Ectodomain of LDL Receptor-related Protein 1 (LRP1) Unravels pH-dependent Conformational Changes and the Stoichiometry of Binding with Receptor-associated Protein (RAP).The Journal of biological chemistry · 2017Article
- Low Density Lipoprotein Receptor Related Proteins as Regulators of Neural Stem and Progenitor Cell Function.Stem cells international · 2016Review
- Myosin-1c regulates the dynamic stability of E-cadherin-based cell-cell contacts in polarized Madin-Darby canine kidney cells.Molecular biology of the cell · 2013Article
- Receptor-associated protein blocks internalization and cytotoxicity of myeloma light chain in cultured human proximal tubular cells.PloS one · 2013Article
- Low density lipoprotein receptor-related protein 1 dependent endosomal trapping and recycling of apolipoprotein E.PloS one · 2012Article
- OCRL controls trafficking through early endosomes via PtdIns4,5P₂-dependent regulation of endosomal actin.The EMBO journal · 2011Article
- Effects of the prosegment and pH on the activity of PCSK9: evidence for additional processing events.The Journal of biological chemistry · 2010Article
- Oral administration of a GSK3 inhibitor increases brain insulin-like growth factor I levels.The Journal of biological chemistry · 2010Article
- Ligand-modified gene carriers increased uptake in target cells but reduced DNA release and transfection efficiency.Nanomedicine : nanotechnology, biology, and medicine · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Megalin (gp330) is a member of the low-density lipoprotein receptor gene family. Like other members of the family, it is an endocytic receptor that binds a number of specific ligands. Megalin also binds the receptor-associated protein (RAP) that serves as an exocytic traffic chaperone and inhibits ligand binding to the receptor. To investigate the fate of megalin/RAP complexes, we bound RAP glutathione-S-transferase fusion protein (RAP-GST) to megalin at the surface of L2 yolk sac carcinoma cells and followed the trafficking of the complexes by immunofluorescence and immunogold labeling and by their distribution on Percoll gradients. We show that megalin/RAP-GST complexes, which are internalized via clathrin-coated pits, are delivered to early endosomes where they accumulate during an 18 degrees C temperature block and colocalize with transferrin and transferrin receptor. Upon release from the temperature block, the complexes travel to late endosomes where they colocalize with rab7 and can be coprecipitated with anti-RAP-GST antibodies. Dissociation of the complex occurs in late endosomes and is most likely triggered by the low pH (approximately 5.5) of this compartment. RAP is then rapidly delivered to lysosomes and degraded whereas megalin is recycled to the cell surface. When the ligand, lipoprotein lipase, was bound to megalin, the receptor was found to recycle through early endosomes. We conclude that in contrast to receptor/ligand complexes, megalin/RAP complexes traffic through late endosomes, which is a novelty for members of the low-density lipoprotein receptor gene family.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.