ArticleMolecular biology of the cell1997
PF20 gene product contains WD repeats and localizes to the intermicrotubule bridges in Chlamydomonas flagella.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 108 citations in OpenAlex.
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- The Chlamydomonas Genome Project, version 6: Reference assemblies for mating-type plus and minus strains reveal extensive structural mutation in the laboratory.The Plant cell · 2023Article
- A look under the hood of the machine that makes cilia beat.Nature structural & molecular biology · 2022Article
- Cryo-EM structure of an active central apparatus.Nature structural & molecular biology · 2022Article
- Heme-binding protein CYB5D1 is a radial spoke component required for coordinated ciliary beating.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Central Apparatus, the Molecular Kickstarter of Ciliary and Flagellar Nanomachines.International journal of molecular sciences · 2021Review
- Motile cilia genetics and cell biology: big results from little mice.Cellular and molecular life sciences : CMLS · 2021Review
- Analysis of the sperm flagellar axoneme using gene-modified mice.Experimental animals · 2020Review
- Asymmetries in the cilia ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Identification and mapping of central pair proteins by proteomic analysis.Biophysics and physicobiology · 2020Article
- Rare Human Diseases: Model Organisms in Deciphering the Molecular Basis of Primary Ciliary Dyskinesia.Cells · 2019Review
- Structural organization of the C1a-e-c supercomplex within the ciliary central apparatus.The Journal of cell biology · 2019Article
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- Proteome of the central apparatus of a ciliary axoneme.The Journal of cell biology · 2019Article
- Elongin B is a binding partner of the male germ cell nuclear speckle protein sperm-associated antigen 16S (SPAG16S) and is regulated post-transcriptionally in the testis.Reproduction, fertility, and development · 2019Article
- The Central Apparatus of Cilia and Eukaryotic Flagella.Cold Spring Harbor perspectives in biology · 2017Review
- Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5.BMC molecular biology · 2017Article
- Developmental change in translation initiation alters the localization of a common microbial protein necessary for Toxoplasma chronic infection.Molecular microbiology · 2016Article
- Patching Holes in the Chlamydomonas Genome.G3 (Bethesda, Md.) · 2016Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
The central pair of microtubules and their associated structures play a significant role in regulating flagellar motility. To begin a molecular analysis of these components, we generated central apparatus-defective mutants in Chlamydomonas reinhardtii using insertional mutagenesis. One paralyzed mutant recovered in our screen contains an allele of a previously identified mutation, pf20. Mutant cells have paralyzed flagella, and the entire central apparatus is missing in isolated axonemes. We have cloned the wild-type PF20 gene and confirmed its identity by rescuing the pf20 mutant phenotype upon transformation. Rescued transformants were wild type in motility and in axonemal ultrastructure. A cDNA clone containing a single, long open reading frame was obtained and sequenced. Database searches using the predicted 606-amino acid sequence of PF20 indicate that the protein contains five contiguous WD repeats. These repeats are found in a number of proteins with diverse cellular functions including beta-transducin and dynein intermediate chains. An antibody was raised against a fusion protein expressed from the cloned cDNA. Immunogold labeling of wild-type axonemes indicates that the PF20 protein is localized along the length of the C2 microtubule on the intermicrotubule bridges connecting the two central microtubules. We suggest that the PF20 gene product is a new member of the family of WD repeat proteins and is required for central microtubule assembly and/or stability and flagellar motility.
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