ArticleMolecular biology of the cell1997
Phorbol 12-myristate 13-acetate down-regulates Na,K-ATPase independent of its protein kinase C site: decrease in basolateral cell surface area.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 38 citations in OpenAlex.
- Myosin IIB isoform plays an essential role in the formation of two distinct types of macropinosomes.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Dopamine receptors and hypertension.Current hypertension reports · 2008Review
- Fetal brain hypometabolism during prolonged hypoxaemia in the llama.The Journal of physiology · 2005Article
- Molecular basis for the inhibition of G protein-coupled inward rectifier K(+) channels by protein kinase C.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Angiotensin II AT1 receptor stimulates Na+ -K+ATPase activity through a pathway involving PKC-zeta in rat thyroid cells.The Journal of physiology · 2003Article
- Dopamine-induced exocytosis of Na,K-ATPase is dependent on activation of protein kinase C-epsilon and -delta.Molecular biology of the cell · 2002Article
- Cyclic AMP increases cell surface expression of functional Na,K-ATPase units in mammalian cortical collecting duct principal cells.Molecular biology of the cell · 2001Article
- Specific and nonspecific effects of protein kinase C on the epithelial Na (+) channel.The Journal of general physiology · 2000Article
- Is phosphorylation of the alpha1 subunit at Ser-16 involved in the control of Na,K-ATPase activity by phorbol ester-activated protein kinase C?Molecular biology of the cell · 2000Article
- Insulin-induced stimulation of Na+,K(+)-ATPase activity in kidney proximal tubule cells depends on phosphorylation of the alpha-subunit at Tyr-10.Molecular biology of the cell · 1999Article
- [Ca2+]i determines the effects of protein kinases A and C on activity of rat renal Na+,K+-ATPase.The Journal of physiology · 1999Article
- Regulation of Na+ pump function by aldosterone is alpha-subunit isoform specific.The Journal of physiology · 1999Article
- Adenosine inhibits the transfected Na+-H+ exchanger NHE3 in Xenopus laevis renal epithelial cells (A6/C1).The Journal of physiology · 1999Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effect of protein kinase C (PKC) stimulation on the pump current (Ip) generated by the Na,K-ATPase was measured in A6 epithelia apically permeabilized with amphotericin B. Phorbol 12-myristate 13-acetate (PMA) produced a decrease in Ip carried by sodium pumps containing the endogenous Xenopus laevis or transfected Bufo marinus alpha 1 subunits (approximately 30% reduction within 25 min, maximum after 40 min) independent of the PKC phosphorylation site (T15A/S16A). In addition to this major effect of PMA, which was independent of the intracellular sodium concentration and was prevented by the PKC inhibitor bisindolylmaleimide GF 109203X (BIM), another BIM-resistant, PKC site-independent decrease was observed when the Ip was measured at low sodium concentrations (total reduction approximately 50% at 5 mM sodium). Using ouabain binding and cell surface biotinylation, stimulation of PKC was shown to reduce surface Na,K-ATPase by 14 to 20% within 25 min. The same treatment stimulated fluid phase endocytosis sevenfold and decreased by 16.5% the basolateral cell surface area measured by transepithelial capacitance measurements. In conclusion, PKC stimulation produces a decrease in sodium pump function which can be attributed, to a large extent, to a withdrawal of sodium pumps from the basolateral cell surface independent of their PKC site. This reduction of the number of sodium pumps is parallel to a decrease in basolateral membrane area.
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