ArticleNucleic acids research1997
Efficient priming of PCR with short oligonucleotides conjugated to a minor groove binder.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
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Who cites it
54 citing papers in PubMed, 204 citations in OpenAlex.
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- Ameliorative Effects of α-Tocopherol and/or Coenzyme Q10 on Phenytoin-Induced Cognitive Impairment in Rats: Role of VEGF and BDNF-TrkB-CREB Pathway.Neurotoxicity research · 2019Article
- Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test.Scientific reports · 2019Article
- Assessment of tumor necrosis factor alpha polymorphism TNF-αMolecular biology reports · 2018Article
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- Speeding up biomolecular interactions by molecular sledding.Chemical science · 2016Article
- Novel Mutations in the Voltage-Gated Sodium Channel of Pyrethroid-Resistant Varroa destructor Populations from the Southeastern USA.PloS one · 2016Article
- Evolutionary and functional novelty of pancreatic ribonuclease: a study of Musteloidea (order Carnivora).Scientific reports · 2014Article
- Combination of allele-specific detection techniques to quantify minority resistance variants in hepatitis B infection: a novel approach.Journal of virological methods · 2013Article
- An amino acid substitution (L925V) associated with resistance to pyrethroids in Varroa destructor.PloS one · 2013Article
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- Association of DDAH2 gene polymorphism with cardiovascular disease in Egyptian patients.Journal of genetics · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The tripeptide 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI3) binds to the minor groove of DNA with high affinity. When this minor groove binder (MGB) is conjugated to the 5'-end of short oligodeoxynucleotides (ODNs), the conjugates form unusually stable hybrids with complementary DNA in which the tethered CDPI3group resides in the minor groove. We show that these conjugates can be used as PCR primers. Due to their unusually high binding affinity, conjugates as short as 8-10mers can be used to amplify DNA with good specificity and efficiency. The reduced length primers described here might be appropriate for the PCR amplification of viral sequences which possess a high degree of variability (e.g., HPV, HIV) or for recent techniques such as gene hunting and differential display which amplify multiple sequences using short primer pairs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.