ArticleNucleic acids research1997
sigma factor mutations affecting the sequence-selective interaction of RNA polymerase with -10 region single-stranded DNA.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Tuning theJournal of bacteriology · 2019Article
- Region 4 of Rhizobium etli Primary Sigma Factor (SigA) Confers Transcriptional Laxity in Escherichia coli.Frontiers in microbiology · 2016Article
- Promoter spacer DNA plays an active role in integrating the functional consequences of RNA polymerase contacts with -10 and -35 promoter elements.Biophysical chemistry · 2011Article
- Effects of substitutions at position 180 in the Escherichia coli RNA polymerase σ 70 subunit.Journal of biosciences · 2011Article
- The core-independent promoter-specific interaction of primary sigma factor.Nucleic acids research · 2011Article
- Folded DNA in action: hairpin formation and biological functions in prokaryotes.Microbiology and molecular biology reviews : MMBR · 2010Review
- Promoter recognition by bacterial alternative sigma factors: the price of high selectivity?Genes & development · 2009Article
- Regularities in the E. coli promoters composition in connection with the DNA strands interaction and promoter activity.Journal of Zhejiang University. Science. B · 2006Article
- Using disulfide bond engineering to study conformational changes in the beta'260-309 coiled-coil region of Escherichia coli RNA polymerase during sigma(70) binding.Journal of bacteriology · 2002Article
- Two "wild-type" variants of Escherichia coli sigma(70): context-dependent effects of the identity of amino acid 149.Journal of bacteriology · 2002Article
- Escherichia coli promoter opening and -10 recognition: mutational analysis of sigma70.The EMBO journal · 2000Article
- Effects of amino acid substitutions at conserved and acidic residues within region 1.1 of Escherichia coli sigma(70).Journal of bacteriology · 2000Article
- A mutation in region 1.1 of sigma70 affects promoter DNA binding by Escherichia coli RNA polymerase holoenzyme.The EMBO journal · 1999Article
- RNA polymerase-promoter interactions: the comings and goings of RNA polymerase.Journal of bacteriology · 1998Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Thesigmasubunit of RNA polymerase determines promoter recognition and catalyzes DNA strand separation. The -35 promoter region is recognized by a helix-turn-helix motif in region 4, while the -10 region is specified, at least in part, by an amphipathic helix in region 2. We have proposed that conserved aromatic residues insigmaregion 2.3 interact with the non-template strand of the -10 element to drive open complex formation. We now report that Bacillus subtilis sigmaA holoenzyme, but neither core nor sigmaA alone, binds with high selectivity to single-stranded (ss) DNA containing the non-template -10 consensus sequence. UV irradiation of holoenzyme-ssDNA complexes efficiently crosslinks sigmaA to DNA and protease mapping supports a primary contact site in or near region 2. Several mutations in sigmaA region 2.3, shown previously to impair promoter melting, affect ssDNA binding: Y184A decreases binding selectivity, while Y189A and W193A decrease the efficiency of photocrosslinking. These results support a model in which these aromatic amino acids are juxtaposed to ssDNA, consistent with their demonstrated role in stabilizing the open complex.
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Registered trials
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