ArticleThe EMBO journal1997
Cell-free synthesis and assembly of connexins into functional gap junction membrane channels.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed, 160 citations in OpenAlex.
- Intercellular Communication via Mitotic Nanotubes is Influenced by Connexin-43 Trafficking and Actin Remodeling.bioRxiv : the preprint server for biology · 2026Article
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- Oligomerization of the heteromeric γ-aminobutyric acid receptor GABAScientific reports · 2022Article
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- The Role of Connexin 43 in Lung Disease.Life (Basel, Switzerland) · 2020Review
- Innexins: Expression, Regulation, and Functions.Frontiers in physiology · 2018Review
- The electrical synapse: Molecular complexities at the gap and beyond.Developmental neurobiology · 2017Review
- Cardiac Cx43, Cx40 and Cx45 co-assembling: involvement of connexins epitopes in formation of hemichannels and Gap junction channels.BMC cell biology · 2017Review
- Functional analysis of a nonsyndromic hearing loss-associated mutation in the transmembrane II domain of the GJC3 gene.International journal of medical sciences · 2017Article
- Molecular mechanisms regulating formation, trafficking and processing of annular gap junctions.BMC cell biology · 2016Review
- Cell-free expression of a functional pore-only sodium channel.Protein expression and purification · 2015Article
- Expression of a mitochondrial progesterone receptor (PR-M) in leiomyomata and association with increased mitochondrial membrane potential.The Journal of clinical endocrinology and metabolism · 2014Article
- Cxs and Panx- hemichannels in peripheral and central chemosensing in mammals.Frontiers in cellular neuroscience · 2014Review
- A truncated progesterone receptor (PR-M) localizes to the mitochondrion and controls cellular respiration.Molecular endocrinology (Baltimore, Md.) · 2013Article
- A cataract-causing connexin 50 mutant is mislocalized to the ER due to loss of the fourth transmembrane domain and cytoplasmic domain.FEBS open bio · 2013Article
- Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes.Scientific reports · 2013Article
- Gap junctions.Comprehensive Physiology · 2012Review
- Posttranslational modifications in connexins and pannexins.The Journal of membrane biology · 2012Review
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Several different gap junction channel subunit isotypes, known as connexins, were synthesized in a cell-free translation system supplemented with microsomal membranes to study the mechanisms involved in gap junction channel assembly. Previous results indicated that the connexins were synthesized as membrane proteins with their relevant transmembrane topology. An integrated biochemical and biophysical analysis indicated that the connexins assembled specifically with other connexin subunits. No interactions were detected between connexin subunits and other co-translated transmembrane proteins. The connexins that were integrated into microsomal vesicles assembled into homo- and hetero-oligomeric structures with hydrodynamic properties of a 9S particle, consistent with the properties reported for hexameric gap junction connexons derived from gap junctions in vivo. Further, cell-free assembled homo-oligomeric connexons composed of beta1 or beta2 connexin were reconstituted into synthetic lipid bilayers. Single channel conductances were recorded from these bilayers that were similar to those measured for these connexons produced in vivo. Thus, this is the first direct evidence that the synthesis and assembly of a gap junction connexon can take place in microsomal membranes. Finally, the cell-free system has been used to investigate the properties of alpha1, beta1 and beta2 connexin to assemble into hetero-oligomers. Evidence has been obtained for a selective interaction between individual connexin isotypes and that a signal determining the potential hetero-oligomeric combinations of connexin isotypes may be located in the N-terminal sequence of the connexins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.