ArticleThe EMBO journal1997
New structure and function in plant K+ channels: KCO1, an outward rectifier with a steep Ca2+ dependency.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 154 citations in OpenAlex.
- Research Progress on Plant Shaker KPlants (Basel, Switzerland) · 2024Review
- Homologous Cloning of Potassium Channel Genes From the Superior Apple Rootstock Line 12-2, Which is Tolerant to Apple Replant Disease.Frontiers in genetics · 2022Article
- Recent Advances in Genome-wide Analyses of Plant Potassium Transporter Families.Current genomics · 2021Review
- The uncharacterized geneProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Global Plant Stress Signaling: Reactive Oxygen Species at the Cross-Road.Frontiers in plant science · 2016Review
- Near-isogenic lines of Triticum aestivum with distinct modes of resistance exhibit dissimilar transcriptional regulation during Diuraphis noxia feeding.Biology open · 2014Article
- Strategies for improving potassium use efficiency in plants.Molecules and cells · 2014Review
- Gene expression analysis of rice seedling under potassium deprivation reveals major changes in metabolism and signaling components.PloS one · 2013Article
- The role of K(+) channels in uptake and redistribution of potassium in the model plant Arabidopsis thaliana.Frontiers in plant science · 2013Article
- Assembly and sorting of the tonoplast potassium channel AtTPK1 and its turnover by internalization into the vacuole.Plant physiology · 2011Article
- Competition between uptake of ammonium and potassium in barley and Arabidopsis roots: molecular mechanisms and physiological consequences.Journal of experimental botany · 2010Article
- The mechano-gated K(2P) channel TREK-1.European biophysics journal : EBJ · 2009Review
- Heterelogous expression of plant genes.International journal of plant genomics · 2009Article
- KT/HAK/KUP potassium transporters gene family and their whole-life cycle expression profile in rice (Oryza sativa).Molecular genetics and genomics : MGG · 2008Article
- Ca-Responsive cis-Elements in Plants.Plant signaling & behavior · 2007Article
- Rapid, futile K+ cycling and pool-size dynamics define low-affinity potassium transport in barley.Plant physiology · 2006Article
- Interactive domains between pore loops of the yeast K+ channel TOK1 associate with extracellular K+ sensitivity.The Biochemical journal · 2006Article
- Homology modeling identifies C-terminal residues that contribute to the Ca2+ sensitivity of a BKCa channel.Biophysical journal · 2005Article
- In the yeast potassium channel, Tok1p, the external ring of aspartate residues modulates both gating and conductance.Pflugers Archiv : European journal of physiology · 2005Article
- TPK1 is a vacuolar ion channel different from the slow-vacuolar cation channel.Plant physiology · 2005Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Potassium (K+) channels mediating important physiological functions are characterized by a common pore-forming (P) domain. We report the cloning and functional analysis of the first higher plant outward rectifying K+ channel (KCO1) from Arabidopsis thaliana. KCO1 belongs to a new class of 'two-pore' K+ channels recently described in human and yeast. KCO1 has four putative transmembrane segments and tandem calcium-binding EF-hand motifs. Heterologous expression of KCO1 in baculovirus-infected insect (Spodoptera frugiperda) cells resulted in outwardly rectifying, K+-selective currents elicited by depolarizing voltage pulses in whole-cell measurements. Activation of KCO1 was strongly dependent on the presence of nanomolar concentrations of cytosolic free Ca2+ [Ca2+]cyt. No K+ currents were detected when [Ca2+]cyt was adjusted to <150 nM. However, KCO1 strongly activated at increasing [Ca2+]cyt, with a saturating activity observed at approximately 300 nM [Ca2+]cyt. KCO1 single channel analysis on excised membrane patches, resulting in a single channel conductance of 64 pS, confirmed outward rectification as well as Ca2+-dependent activation. These data suggest a direct link between calcium-mediated signaling processes and K+ ion transport in higher plants. The identification of KCO1 as the first plant K+ outward channel opens a new field of structure-function studies in plant ion channels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.