ArticleThe Biochemical journal1997
Purification of a dichlorophenol-indophenol oxidoreductase from rat and bovine synaptic membranes: tight complex association of a glyceraldehyde-3-phosphate dehydrogenase isoform, TOAD64, enolase-gamma and aldolase C.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 56 citations in OpenAlex.
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- Impact of Autoantibodies against Glycolytic Enzymes on Pathogenicity of Autoimmune Retinopathy and Other Autoimmune Disorders.Frontiers in immunology · 2017Review
- Significance of Anti-retinal Autoantibodies in Cancer-associated Retinopathy with Gynecological Cancers.Journal of clinical & experimental ophthalmology · 2013Article
- Hypobaric hypoxia and reoxygenation induce proteomic profile changes in the rat brain cortex.Neuromolecular medicine · 2013Article
- Proteomic identification of protein targets for 15-deoxy-Δ(12,14)-prostaglandin J2 in neuronal plasma membrane.PloS one · 2011Article
- Multifunctional roles of enolase in Alzheimer's disease brain: beyond altered glucose metabolism.Journal of neurochemistry · 2009Review
- Proteomics in animal models of Alzheimer's and Parkinson's diseases.Ageing research reviews · 2009Review
- Redox proteomics in some age-related neurodegenerative disorders or models thereof.NeuroRx : the journal of the American Society for Experimental NeuroTherapeutics · 2006Review
- Critical role of collapsin response mediator protein-associated molecule CRAM for filopodia and growth cone development in neurons.Molecular biology of the cell · 2005Article
- Collapsin response mediator proteins (CRMPs): involvement in nervous system development and adult neurodegenerative disorders.Molecular neurobiology · 2003Review
- Innate differences in protein expression in the nucleus accumbens and hippocampus of inbred alcohol-preferring and -nonpreferring rats.Proteomics · 2003Article
- Collapsin response mediator protein-1: a novel invasion-suppressor gene.Clinical & experimental metastasis · 2003Review
- Isolation and expression pattern of human Unc-33-like phosphoprotein 6/collapsin response mediator protein 5 (Ulip6/CRMP5): coexistence with Ulip2/CRMP2 in Sema3a- sensitive oligodendrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Differential protein S-thiolation of glyceraldehyde-3-phosphate dehydrogenase isoenzymes influences sensitivity to oxidative stress.Molecular and cellular biology · 1999Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NADH-dichlorophenol-indophenol oxidoreductases (PMOs) were purified from synaptic plasma membranes or synaptic vesicles (small recycling vesicles) from both bovine and rat brains and from a neuroblastoma cell line, NB41A3. Several isoforms could be identified in purified plasma membranes and vesicles. Purification of the enzyme activity involved protein extraction with detergents, (NH4)2SO4 precipitation, chromatography under stringent conditions and native PAGE. PMO activity could be attributed to a very tight complex of several proteins that could not be separated except by SDS/PAGE. SDS/PAGE resolved the purified complex into at least five proteins, which could be micro-sequenced and identified unambiguously as hsc70, TOAD64 and glyceraldehyde-3-phosphate dehydrogenase tightly associated with the brain-specific proteins aldolase C and enolase-gamma. Enzyme activity could be purified from both synaptic plasma membranes and recycling vesicles, yields being much greater from the latter source. Highly purified plasma membranes (prepared from a neuroblastoma cell line NB41A3 by iminobiotinylation of intact cells and affinity purification with avidin and anti-avidin antibodies under very stringent conditions) also displayed PMO activity tightly associated with TOAD64. The association of PMO in a tight complex was confirmed by its immunoprecipitation from cellular and membrane extracts of NB41A3 using antibodies directed against any component protein of the complex followed by immunodetection with antibodies directed against the other members. Antibodies also inhibited the enzyme activity synergistically. In addition, induction of the different components of the complex during dichlorophenol-indophenol stress was demonstrated by the S1 RNase-protection assay in synchronized NB41A3 cells. The role of the complex in membrane fusion and cellular response to extracellular oxidative stress during growth and development is discussed.
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