ArticleThe Biochemical journal1997
Sphingosine 1-phosphate stimulates rho-mediated tyrosine phosphorylation of focal adhesion kinase and paxillin in Swiss 3T3 fibroblasts.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 112 citations in OpenAlex.
- Targeting Sphingosine-1-Phosphate Signaling in Breast Cancer.International journal of molecular sciences · 2024Review
- Dialogue between VE-Cadherin and Sphingosine 1 Phosphate Receptor1 (S1PR1) for Protecting Endothelial Functions.International journal of molecular sciences · 2023Review
- Slit-Robo signalling establishes a Sphingosine-1-phosphate gradient to polarise fin mesenchyme.EMBO reports · 2022Article
- Sex Differences in Cardiovascular Diseases: A Matter of Estrogens, Ceramides, and Sphingosine 1-Phosphate.International journal of molecular sciences · 2022Review
- Overexpression of sphingosine-1-phosphate lyase protects insulin-secreting cells against cytokine toxicity.The Journal of biological chemistry · 2017Article
- Sphingosine Kinases and Sphingosine 1-Phosphate Receptors: Signaling and Actions in the Cardiovascular System.Frontiers in pharmacology · 2017Review
- Role played by paxillin and paxillin tyrosine phosphorylation in hepatocyte growth factor/sphingosine-1-phosphate-mediated reactive oxygen species generation, lamellipodia formation, and endothelial barrier function.Pulmonary circulation · 2015Article
- Podocyte pathology and nephropathy - sphingolipids in glomerular diseases.Frontiers in endocrinology · 2014Review
- Regulation of cytoskeleton organization by sphingosine in a mouse cell model of progressive ovarian cancer.Biomolecules · 2013Article
- SHEP1 partners with CasL to promote marginal zone B-cell maturation.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Sphingosine-1-phosphate signaling in vasculogenesis and angiogenesis.World journal of biological chemistry · 2010Article
- S1P metabolism in cancer and other pathological conditions.Biochimie · 2010Review
- Serine palmitoyltransferase subunit 1 is present in the endoplasmic reticulum, nucleus and focal adhesions, and functions in cell morphology.Biochimica et biophysica acta · 2009Article
- Sphingosine-1-phosphate receptor signalling in the heart.Cardiovascular research · 2009Review
- Anti-sphingosine-1-phosphate monoclonal antibodies inhibit angiogenesis and sub-retinal fibrosis in a murine model of laser-induced choroidal neovascularization.Experimental eye research · 2009Article
- Sphingosine 1-phosphate potentiates human lung fibroblast chemotaxis through the S1P2 receptor.American journal of respiratory cell and molecular biology · 2008Article
- The G12/13-RhoA signaling pathway contributes to efficient lysophosphatidic acid-stimulated cell migration.Oncogene · 2006Article
- Tumor necrosis factor-alpha induces stress fiber formation through ceramide production: role of sphingosine kinase.Molecular biology of the cell · 2001Article
- Sphingosine 1-phosphate signalling in mammalian cells.The Biochemical journal · 2000Review
- Sphingoid base synthesis requirement for endocytosis in Saccharomyces cerevisiae.The EMBO journal · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
Sphingosine 1-phosphate (SPP), a sphingolipid second messenger implicated in the mitogenic action of platelet-derived growth factor [Olivera, A. and Spiegel, S. (1993) Nature (London) 365, 557-560], induced rapid reorganization of the actin cytoskeleton resulting in stress-fibre formation. SPP also induced transient tyrosine phosphorylation of focal adhesion kinase (p125(FAK)), a cytosolic tyrosine kinase that localizes in focal adhesions, and of the cytoskeleton-associated protein paxillin. Exoenzyme C3 transferase, which ADP-ribosylates Rho (a Ras-related small GTP binding protein) on asparagine-41 and renders it biologically inactive, inhibited both stress-fibre formation and protein tyrosine phosphorylation induced by SPP. Thus Rho may be an upstream regulator of both stress-fibre formation and tyrosine phosphorylation of p125(FAK) and paxillin. Pretreatment with PMA, an activator of protein kinase C (PKC), inhibited the stimulation of stress-fibre formation induced by 1-oleoyl-lysophosphatidic acid (LPA) but not that by SPP. Similarly, PMA also decreased LPA-induced tyrosine phosphorylation of p125(FAK) and paxillin without abrogating the response to SPP. Thus PKC is involved in LPA- but not SPP-dependent signalling. The polyanionic drug suramin, a broad-specificity inhibitor of ligand-receptor interactions, did not inhibit either the mitogenic effect of SPP or its stimulation of tyrosine phosphorylation of p125(FAK). However, suramin markedly inhibited these responses induced by LPA. These results suggest that in contrast with LPA, SPP may be acting intracellularly in Swiss 3T3 fibroblasts to stimulate tyrosine phosphorylation of p125(FAK) and paxillin and cell growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.