ArticleThe Journal of cell biology1997
Partitioning of the Golgi apparatus during mitosis in living HeLa cells.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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Who cites it
102 citing papers in PubMed, 216 citations in OpenAlex.
- Structure-Activity Relationship of Cycling Molecular Assemblies for Golgi Targeting and Disruption.Journal of medicinal chemistry · 2026Article
- Mitotic Cdc42 waves encode PI(3,4)PScience advances · 2026Article
- Organelles harbour pH gradients.Research square · 2026Article
- Organelles harbour pH gradients.bioRxiv : the preprint server for biology · 2025Article
- Cell cycle-regulated tug-of-war between microtubule motors positions major trafficking organelles.bioRxiv : the preprint server for biology · 2025Article
- Cargoes move from cis to trans-Golgi compartments and concentrate in the TGN before exiting.EMBO reports · 2025Article
- Vimentin intermediate filaments provide structural stability to the mammalian Golgi complex.Journal of cell science · 2023Article
- Specific association of TBK1 with the trans-Golgi network following STING stimulation.Cell structure and function · 2022Article
- Importin α phosphorylation promotes TPX2 activation by GM130 to control astral microtubules and spindle orientation.Journal of cell science · 2021Article
- Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.The Journal of cell biology · 2021Article
- The Role of GM130 in Nervous System Diseases.Frontiers in neurology · 2021Review
- Article
- Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-α.Nature communications · 2019Article
- Golgin45-Syntaxin5 Interaction Contributes to Structural Integrity of the Golgi Stack.Scientific reports · 2019Article
- Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors.BMC biology · 2018Article
- Golgi ribbon disassembly during mitosis, differentiation and disease progression.Current opinion in cell biology · 2017Review
- Cruising the cellular highways: How human papillomavirus travels from the surface to the nucleus.Virus research · 2017Review
- Organelles - understanding noise and heterogeneity in cell biology at an intermediate scale.Journal of cell science · 2017Review
- Mitotic Golgi disassembly is required for bipolar spindle formation and mitotic progression.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Growth of the Mammalian Golgi Apparatus during Interphase.Molecular and cellular biology · 2016Article
42 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Golgi apparatus of HeLa cells was fluorescently tagged with a green fluorescent protein (GFP), localized by attachment to the NH2-terminal retention signal of N-acetylglucosaminyltransferase I (NAGT I). The location was confirmed by immunogold and immunofluorescence microscopy using a variety of Golgi markers. The behavior of the fluorescent Golgi marker was observed in fixed and living mitotic cells using confocal microscopy. By metaphase, cells contained a constant number of Golgi fragments dispersed throughout the cytoplasm. Conventional and cryoimmunoelectron microscopy showed that the NAGT I-GFP chimera (NAGFP)-positive fragments were tubulo-vesicular mitotic Golgi clusters. Mitotic conversion of Golgi stacks into mitotic clusters had surprisingly little effect on the polarity of Golgi membrane markers at the level of fluorescence microscopy. In living cells, there was little self-directed movement of the clusters in the period from metaphase to early telophase. In late telophase, the Golgi ribbon began to be reformed by a dynamic process of congregation and tubulation of the newly inherited Golgi fragments. The accuracy of partitioning the NAGFP-tagged Golgi was found to exceed that expected for a stochastic partitioning process. The results provide direct evidence for mitotic clusters as the unit of partitioning and suggest that precise regulation of the number, position, and compartmentation of mitotic membranes is a critical feature for the ordered inheritance of the Golgi apparatus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.