ArticleAnalytical biochemistry1997
Long-lifetime metal-ligand pH probe.
Article in Analytical biochemistry, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- FITC and Ru(phen)32+ co-doped silica particles as visualized ratiometric pH indicator.Nanoscale research letters · 2011Article
- Measurement of the Intensity of Long-Lifetime Luminophores in the Presence of Background Signals Using Phase-Modulation Fluorometry.Applied spectroscopy · 1999Article
- Polarization sensing with visual detection.Analytical chemistry · 1999Article
- Synthesis and spectral characterization of a thiol-reactive long-lifetime Ru(II) complex.Analytical biochemistry · 1997Article
- Pyrazole-substituted near-infrared cyanine dyes exhibit pH-dependent fluorescence lifetime properties.Photochemistry and photobiologyArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We describe the synthesis and fluorescence spectral characterization of a pH-sensitive metal-ligand complex, [Ru(deabpy)(bpy)2]2., where deabpy is 4,4'-diethylaminomethyl-2,2'-bipyridine. This metal-ligand complex (MLC) was found to display pH-dependent intensities, emission spectra, and decay times, with the changes centered near the physiological useful pH value of 7.5. The apparent pKa values were not found to be dependent on ionic strength. The compound was found to be useful for lifetime-based sensing by phase-modulation fluorometry. Global analysis of the intensity decays over a range of pH values revealed two decay times of 235 and 380 ns, associated with the protonated and unprotonated forms, respectively. Because of its long decay time, optical pH measurements could be accomplished by phase-modulation fluorometry with a conveniently low modulation frequency of 700 kHz. The lifetime data were obtained with either a amplitude-modulated laser or with an amplitude-modulated blue-light-emitting diode. This pH-sensitive complex also displays a modest spectral shift with change in pH, allowing its use as a wavelength-ratiometric MLC probe. One can imagine lifetime sensors for a variety of blood cations and point-of-care assays based on long-lifetime metal-ligand complexes and simple solid-state light sources and detectors.
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Registered trials
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