ArticleProceedings of the National Academy of Sciences of the United States of America1997
Specific complex formation between the type II bare lymphocyte syndrome-associated transactivators CIITA and RFX5.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 139 citations in OpenAlex.
- The intrinsically disordered transcriptional activation domain of CIITA is functionally tuneable by single substitutions: An exception or a new paradigm?Protein science : a publication of the Protein Society · 2024Article
- Structural aspects of the MHC expression control system.Biophysical chemistry · 2022Review
- Case Report: Novel splicing mutations inFrontiers in genetics · 2022Article
- The MHC Class II Transactivator CIITA: Not (Quite) the Odd-One-Out Anymore among NLR Proteins.International journal of molecular sciences · 2021Review
- Comprehensive Review of Steroid-Sensitive Nephrotic Syndrome Genetic Risk Loci and Transcriptional Regulation as a Possible Mechanistic Link to Disease Risk.Kidney international reports · 2021Article
- PYHIN1 regulates pro-inflammatory cytokine induction rather than innate immune DNA sensing in airway epithelial cells.The Journal of biological chemistry · 2020Article
- Regulatory factor X5 promotes hepatocellular carcinoma progression by transactivating tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta and suppressing apoptosis.Chinese medical journal · 2019Article
- Identification of four differentially methylated genes as prognostic signatures for stage I lung adenocarcinoma.Cancer cell international · 2018Article
- Clinical, Immunological, and Molecular Findings in Five Patients with Major Histocompatibility Complex Class II Deficiency from India.Frontiers in immunology · 2018Article
- Revisiting the specificity of the MHC class II transactivator CIITA in classical murine dendritic cells in vivo.European journal of immunology · 2017Article
- Nod2: A Critical Regulator of Ileal Microbiota and Crohn's Disease.Frontiers in immunology · 2016Review
- Transcriptional and posttranscriptional regulations of the HLA-G gene.Journal of immunology research · 2014Review
- Regulation of gene expression in HBV- and HCV-related hepatocellular carcinoma: integrated GWRS and GWGS analyses.International journal of clinical and experimental medicine · 2014Article
- Interferon-γ resets muscle cell fate by stimulating the sequential recruitment of JARID2 and PRC2 to promoters to repress myogenesis.Science signaling · 2013Article
- NLRC5 cooperates with the RFX transcription factor complex to induce MHC class I gene expression.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Gamma interferon modulates myogenesis through the major histocompatibility complex class II transactivator, CIITA.Molecular and cellular biology · 2011Article
- Solution structure of the heterotrimeric complex between the interaction domains of RFX5 and RFXAP from the RFX gene regulatory complex.Journal of molecular biology · 2010Article
- Formation of the RFX gene regulatory complex induces folding of the interaction domain of RFXAP.Proteins · 2009Article
- Mitogen-activated protein kinase ERK1/2 regulates the class II transactivator.The Journal of biological chemistry · 2008Article
- The zinc finger proteins ZXDA and ZXDC form a complex that binds CIITA and regulates MHC II gene transcription.Journal of molecular biology · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Two of the genes defective in the five complementation groups identified in the class II-negative bare lymphocyte syndrome or corresponding laboratory mutants have been cloned. One gene encodes a protein, RFX5, that is a member of the RFX family of DNA binding proteins. The other, CIITA, encodes a large protein with a defined acidic transcriptional activation domain; this protein does not interact with DNA. Expression plasmids encoding regions of RFX5 fused to the GAL4 DNA binding domain activated transcription from a reporter construct containing GAL4 sites in a cotransfection assay in the Raji human B cell line. However, these plasmids produced transcriptional activity in HeLa cells only in conjunction with interferon gamma stimulation, a condition in which expression of both CIITA and class II major histocompatibility complex surface proteins are induced. Furthermore, these plasmids were not active in RJ2.2.5, an in vitro mutagenized derivative of Raji in which both copies of CIITA are defective. Transcriptional activation by the RFX5 fusion protein could be restored in RJ2.2.5 by cotransfection with a CIITA expression plasmid. Finally, a direct interaction between RFX5 and CIITA was detected with the yeast two-hybrid and far-Western blot assays. Thus, RFX5 can activate transcription only in cooperation with CIITA. RFX5 and CIITA associate to form a complex capable of activating transcription from class II major histocompatibility complex promoters. In this complex, promoter specificity is determined by the DNA binding domain of RFX5 and the general transcription apparatus is recruited by the acidic activation domain of CIITA.
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