ArticleProceedings of the National Academy of Sciences of the United States of America1997
Assembly and positioning of microtubule asters in microfabricated chambers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed.
- A centrally positioned cluster of multiple centrioles in antigen-presenting cells fosters T cell activation.Nature communications · 2026Article
- Mechanical self-organization of the actin cytoskeleton in artificial cells.Biophysics and physicobiology · 2026Article
- The dual guardians of cellular stability: exploring nesprin and lamin in senescence.Cell death & disease · 2025Review
- A cytokinetic ring-driven cell rotation achieves Hertwig's rule in early development.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Nesprin proteins: bridging nuclear envelope dynamics to muscular dysfunction.Cell communication and signaling : CCS · 2024Review
- Positioning centrioles and centrosomes.The Journal of cell biology · 2024Review
- Article
- Redundant microtubule crosslinkers prevent meiotic spindle bending to ensure diploid offspring in C. elegans.PLoS genetics · 2023Article
- Tension at intercellular junctions is necessary for accurate orientation of cell division in the epithelium plane.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Actin network architecture can ensure robust centering or sensitive decentering of the centrosome.The EMBO journal · 2022Article
- Dynein-dependent collection of membranes defines the architecture and position of microtubule asters in isolated, geometrically confined volumes of cell-free extracts.Molecular biology of the cell · 2022Article
- Active condensation of filaments under spatial confinement.Frontiers in physics · 2022Article
- Review
- Microtubule-dependent pushing forces contribute to long-distance aster movement and centration inMolecular biology of the cell · 2020Article
- Review
- Prophase-Specific Perinuclear Actin Coordinates Centrosome Separation and Positioning to Ensure Accurate Chromosome Segregation.Cell reports · 2020Article
- Article
- Review
- Cytoskeleton Force Exertion in Bulk Cytoplasm.Frontiers in cell and developmental biology · 2020Review
- Force balances between interphase centrosomes as revealed by laser ablation.Molecular biology of the cell · 2019Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular organization depends on a variety of molecular assembly processes; while some of these have been studied in simplified cell-free systems, others depend on the confined geometry of cells and cannot be reconstructed using bulk techniques. To study the latter processes in vitro, we fabricated microscopic chambers that simulate the closed environment of cells. We used these chambers to study the positioning of microtubule asters. Microtubule assembly alone, without the action of molecular motors, is sufficient to position asters. Asters with short microtubules move toward the position expected from symmetry; however, once the microtubules become long enough to buckle, symmetry is broken. Calculations and experiments show that the bending-energy landscape has multiple minima. Microtubule dynamic instability modifies the landscape over time and allows asters to explore otherwise inaccessible configurations.
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