ArticleThe Biochemical journal1997
Cytosolic phospholipase A2 is coupled to muscarinic receptors in the human astrocytoma cell line 1321N1: characterization of the transducing mechanism.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 64 citations in OpenAlex.
- The utility of (11)C-arachidonate PET to study in vivo dopaminergic neurotransmission in humans.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2012Trial
- Specific Phospholipid Modulation by Muscarinic Signaling in a Rat Lesion Model of Alzheimer's Disease.ACS chemical neuroscience · 2021Article
- D2-like receptor activation does not initiate a brain docosahexaenoic acid signal in unanesthetized rats.BMC neuroscience · 2014Article
- Translational studies on regulation of brain docosahexaenoic acid (DHA) metabolism in vivo.Prostaglandins, leukotrienes, and essential fatty acids · 2013Review
- Fifteen weeks of dietary n-3 polyunsaturated fatty acid deprivation increase turnover of n-6 docosapentaenoic acid in rat-brain phospholipids.Biochimica et biophysica acta · 2012Article
- Imaging brain signal transduction and metabolism via arachidonic and docosahexaenoic acid in animals and humans.Brain research bulletin · 2012Review
- Docosahexaenoic acid (DHA) incorporation into the brain from plasma, as an in vivo biomarker of brain DHA metabolism and neurotransmission.Prostaglandins & other lipid mediators · 2011Review
- Imaging decreased brain docosahexaenoic acid metabolism and signaling in iPLA(2)β (VIA)-deficient mice.Journal of lipid research · 2010Article
- Bipolar disorder and mechanisms of action of mood stabilizers.Brain research reviews · 2009Review
- Acute but not chronic donepezil increases muscarinic receptor-mediated signaling via arachidonic acid in unanesthetized rats.Journal of Alzheimer's disease : JAD · 2009Article
- Novel translocation responses of cytosolic phospholipase A2alpha fluorescent proteins.Biochimica et biophysica acta · 2008Article
- Imaging apomorphine stimulation of brain arachidonic acid signaling via D2-like receptors in unanesthetized rats.Psychopharmacology · 2008Article
- Flurbiprofen, a cyclooxygenase inhibitor, reduces the brain arachidonic acid signal in response to the cholinergic muscarinic agonist, arecoline, in awake rats.Neurochemical research · 2007Article
- Imaging signal transduction via arachidonic acid in the human brain during visual stimulation, by means of positron emission tomography.NeuroImage · 2007Article
- 5-HT2A/2C receptor signaling via phospholipase A2 and arachidonic acid is attenuated in mice lacking the serotonin reuptake transporter.Psychopharmacology · 2005Article
- Arachidonic acid inhibition of muscarinic receptor-mediated nitric oxide production occurs at the level of calcium mobilization in Chinese hamster ovary cells.Neurochemical research · 2002Article
- Negative regulation of cytosolic phospholipase A(2) by melatonin in the rat pineal gland.The Biochemical journal · 2000Article
- A multiplicity of muscarinic mechanisms: enough signaling pathways to take your breath away.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Thrombin produces phosphorylation of cytosolic phospholipase A2 by a mitogen-activated protein kinase kinase-independent mechanism in the human astrocytoma cell line 1321N1.The Biochemical journal · 1997Article
- Brain metabolism of nutritionally essential polyunsaturated fatty acids depends on both the diet and the liver.Prostaglandins, leukotrienes, and essential fatty acidsArticle
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cholinergic agonist carbachol induced the release of arachidonic acid in the 1321N1 astrocytoma cell line, and this was blocked by atropine, suggesting the involvement of muscarinic receptors. To assess the mechanisms of signalling involved in the response to carbachol, a set of compounds characterized by eliciting responses through different mechanisms was tested. A combination of 4beta-phorbol 12beta-myristate 13alpha-acetate and thapsigargin, an inhibitor of endomembrane Ca2+-ATPase that induces a prolonged elevation of cytosolic Ca2+ concentration, induced an optimal response, suggesting at first glance that both protein kinase C (PKC) and Ca2+ mobilization were involved in the response. This was consistent with the observation that carbachol elicited Ca2+ mobilization and PKC-dependent phosphorylation of cytosolic phospholipase A2 (cPLA2; phosphatide sn-2-acylhydrolase, EC 3.1.1.4) as measured by a decrease in electrophoretic mobility. Nevertheless, the release of arachidonate induced by carbachol was unaltered in media containing decreased concentrations of Ca2+ or in the presence of neomycin, a potent inhibitor of phospholipase C which blocks phosphoinositide turnover and Ca2+ mobilization. Guanosine 5'-[gamma-thio]triphosphate added to the cell-free homogenate induced both [3H]arachidonate release and cPLA2 translocation to the cell membrane fraction in the absence of Ca2+, thus suggesting the existence of an alternative mechanism of cPLA2 translocation dependent on G-proteins and independent of Ca2+ mobilization. From the combination of experiments utilizing biochemical and immunological tools the involvement of cPLA2 was ascertained. In summary, these data indicate the existence in the astrocytoma cell line 1321N1 of a pathway involving the cPLA2 which couples the release of arachidonate to the occupancy of receptors for a neurotransmitter, requires PKC activity and G-proteins and might operate in the absence of Ca2+ mobilization.
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