ArticleBiophysical journal1996
Diffusion of fluorescently labeled macromolecules in cultured muscle cells.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 104 citations in OpenAlex.
- Visualizing sarcomere and cellular dynamics in skeletal muscle to improve cell therapies.eLife · 2024Article
- A Novel Hydrogel Sponge for Three-Dimensional Cell Culture.Pharmaceutics · 2024Article
- Assessing the role of surface layer and molecular probe size in diffusion within meniscus tissue.PloS one · 2024Article
- Vast heterogeneity in cytoplasmic diffusion rates revealed by nanorheology and Doppelgänger simulations.Biophysical journal · 2023Article
- Pore-like diffusion barriers in murine cardiac myocytes.bioRxiv : the preprint server for biology · 2023Article
- The choanoflagellate pore-forming lectin SaroL-1 punches holes in cancer cells by targeting the tumor-related glycosphingolipid Gb3.Communications biology · 2022Article
- [Design, simulation and application of multichannel microfluidic chip for cell migration].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2022Article
- Optofluidic flow meter for sub-nanoliter per minute flow measurements.Journal of biomedical optics · 2022Article
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- Modeling diffusion-based drug release inside a nerve conduit in vitro and in vivo validation study.Drug delivery and translational research · 2021Article
- Membrane Binding of α-Synuclein Stimulates Expansion of SNARE-Dependent Fusion Pore.Frontiers in cell and developmental biology · 2021Article
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- Molecular and macromolecular diffusion in human meniscus: relationships with tissue structure and composition.Osteoarthritis and cartilage · 2020Article
- Chips-on-a-plate device for monitoring cellular migration in a microchannel-based intestinal follicle-associated epithelium model.Biomicrofluidics · 2019Article
- Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells.Nature methods · 2019Article
- Dynamic Measurement of Nanoflows: Analysis and Theory of an Optofluidic Flowmeter.Physical review applied · 2019Article
- Pulmonary Vascular Platform Models the Effects of Flow and Pressure on Endothelial Dysfunction inInternational journal of molecular sciences · 2018Article
- Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots.Nature communications · 2017Article
- Multiscale microenvironmental perturbation of pluripotent stem cell fate and self-organization.Scientific reports · 2017Article
- Stromal Cell-Derived Growth Factor-1 Alpha-Elastin Like Peptide Fusion Protein Promotes Cell Migration and Revascularization of Experimental Wounds in Diabetic Mice.Advances in wound care · 2017Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myotubes were obtained from culture of satellite cells. They had a sarcomeric organization similar to that of muscle. The diffusion in the direction perpendicular to the fibers of microinjected fluorescein isothiocyanate-dextrans of molecular weight ranging from 9500 to 150,000 was examined by modulated fringe pattern photobleaching. On the time scale of the observation, 10-30 S, all of the dextrans were completely mobile in the cytoplasm. The diffusion coefficients were compared to the values obtained in water. The ratio D(cytoplasm)/D(w) decreased with the hydrodynamic radius R(h) of the macromolecules. The mobility of inert molecules in muscle cells is hindered by both the crowding of the fluid phase of the cytoplasm and the screening effect due to myofilaments: D(cytoplasm)/D(w) = (D/D(w)) protein crowding x (D/D(w))(filament screening). The equation (D/D(w))filament screening = exp(-K(L)RCh) was used for the contribution of the filaments to the restriction of diffusion. A free protein concentration of 135 mg/ml, a solvent viscosity of cytoplasm near that of bulk water, and a calculated K(L) of 0.066 nm(-1), which takes into account the sarcomeric organization of filaments, accurately represent our data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.