ArticleBiophysical journal1996
Single nuclear pores visualized by confocal microscopy and image processing.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Pre-ribosomal particles from nucleoli to cytoplasm.Nucleus (Austin, Tex.) · 2024Review
- Pathogenic variants in nucleoporin TPR (translocated promoter region, nuclear basket protein) cause severe intellectual disability in humans.Human molecular genetics · 2022Article
- Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time.Nature communications · 2021Article
- Nuclear Import of Adeno-Associated Viruses Imaged by High-Speed Single-Molecule Microscopy.Viruses · 2021Review
- High-speed super-resolution imaging of rotationally symmetric structures using SPEED microscopy and 2D-to-3D transformation.Nature protocols · 2021Article
- Nuclear export of mRNA molecules studied by SPEED microscopy.Methods (San Diego, Calif.) · 2019Review
- Review
- Review
- Fast and high-accuracy localization for three-dimensional single-particle tracking.Scientific reports · 2013Article
- Three-dimensional distribution of transient interactions in the nuclear pore complex obtained from single-molecule snapshots.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Exploring the nuclear envelope of herpes simplex virus 1-infected cells by high-resolution microscopy.Journal of virology · 2009Article
- Autonomy and robustness of translocation through the nuclear pore complex: a single-molecule study.The Journal of cell biology · 2008Article
- High-resolution near-field optical imaging of single nuclear pore complexes under physiological conditions.Biophysical journal · 2005Article
- Nuclear transport of single molecules: dwell times at the nuclear pore complex.The Journal of cell biology · 2005Article
- Imaging of single-molecule translocation through nuclear pore complexes.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells.The Journal of cell biology · 2001Article
- Visualization and tracking of single protein molecules in the cell nucleus.Biophysical journal · 2001Article
- Kinetic analysis of translocation through nuclear pore complexes.The EMBO journal · 2001Article
- Early localization of NPA58, a rat nuclear pore-associated protein, to the reforming nuclear envelope during mitosis.Journal of biosciences · 2001Article
- Imaging and tracking of single GFP molecules in solution.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How nuclear pore complexes, mediating the transport of nucleic acids, proteins, and metabolites between cell nucleus and cytoplasm, are arranged in the nuclear envelope is essentially unknown. Here we describe a method combining high-resolution confocal imaging with image processing and pattern recognition to visualize single nuclear pore complexes (120 nm diameter), determine their relative positions with nanometer accuracy, and analyze their distribution in situ. The method was tested by means of a model system in which the very same sample areas could be imaged by confocal and electron microscopy. It was thus found that single fluorescent beads of 105 nm nominal diameter could be localized with a lateral accuracy of <20 nm and an axial accuracy of approximately 20 nm. The method was applied to digitonin-permeabilized 3T3 cells, whose nuclear pore complexes were fluorescently labeled with the anti-nucleoporin antibody mAb414. Stacks of optical sections were generated by confocal imaging at high resolution. Herein the nuclear pore complexes appeared as bright diffraction-limited spots whose centers were localized by fitting them by three-dimensional gaussians. The nearest-neighbor distribution function and the pair correlation function were calculated and found to agree well with those of randomly distributed hard cylinders of 138 +/- 17 nm diameter, but not with those of randomly distributed points or nonrandomly distributed cylinders. This was supported by a cluster analysis. Implications for the direct observation of the transport of single particles and molecules through individual nuclear pore complexes are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.