ArticleApplied and environmental microbiology1997
An immunological approach to detect phosphate stress in populations and single cells of photosynthetic picoplankton.
Article in Applied and environmental microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 106 citations in OpenAlex.
- Nutrient-driven regulation of saxitoxin gene expression and toxin production in Raphidiopsis raciborskii (Cyanobacteria).Journal of phycology · 2025Article
- Substrate specificity and ecological significance of PstS homologs in phosphorus uptake in marineMicrobiology spectrum · 2024Article
- Schindler's legacy: from eutrophic lakes to the phosphorus utilization strategies of cyanobacteria.FEMS microbiology reviews · 2022Review
- Why Environmental Biomarkers Work: Transcriptome-Proteome Correlations and Modeling of Multistressor Experiments in the Marine BacteriumJournal of proteome research · 2022Article
- Tools for successful proliferation: diverse strategies of nutrient acquisition by a benthic cyanobacterium.The ISME journal · 2020Article
- Periodic and coordinated gene expression between a diazotroph and its diatom host.The ISME journal · 2019Article
- The elemental composition of virus particles: implications for marine biogeochemical cycles.Nature reviews. Microbiology · 2014Article
- Non-coding RNAs in marine Synechococcus and their regulation under environmentally relevant stress conditions.The ISME journal · 2012Article
- Environmental proteomics of microbial plankton in a highly productive coastal upwelling system.The ISME journal · 2011Article
- Ecological genomics of marine picocyanobacteria.Microbiology and molecular biology reviews : MMBR · 2009Review
- Phosphate acquisition genes in Prochlorococcus ecotypes: evidence for genome-wide adaptation.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Phosphorus scavenging in the unicellular marine diazotroph Crocosphaera watsonii.Applied and environmental microbiology · 2006Article
- Three Prochlorococcus cyanophage genomes: signature features and ecological interpretations.PLoS biology · 2005Article
- Clade-specific 16S ribosomal DNA oligonucleotides reveal the predominance of a single marine Synechococcus clade throughout a stratified water column in the Red Sea.Applied and environmental microbiology · 2003Article
- Identification of a high-affinity phosphate transporter gene in a prasinophyte alga, Tetraselmis chui, and its expression under nutrient limitation.Applied and environmental microbiology · 2003Article
- Detection of the isiA gene across cyanobacterial strains: potential for probing iron deficiency.Applied and environmental microbiology · 2001Article
- Ecological aspects of ntcA gene expression and its use as an indicator of the nitrogen status of marine Synechococcus spp.Applied and environmental microbiology · 2001Article
- Diel rhythms in ribulose-1,5-bisphosphate carboxylase/oxygenase and glutamine synthetase gene expression in a natural population of marine picoplanktonic cyanobacteria (Synechococcus spp.).Applied and environmental microbiology · 1999Article
- Phosphate stress in cultures and field populations of the dinoflagellate prorocentrum minimum detected by a single-cell alkaline phosphatase assayApplied and environmental microbiology · 1999Article
- Niche-partitioning of Prochlorococcus populations in a stratified water column in the eastern North Atlantic Ocean.Applied and environmental microbiology · 1999Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the marine cyanobacterium Synechococcus sp. strain WH7803, PstS is a 32-kDa cell wall-associated phosphate-binding protein specifically synthesized under conditions of restricted inorganic phosphate (P1) availability (D. J. Scanlan, N. H. Mann, and N. G. Carr, Mol. Microbiol. 10:181-191, 1993). We have assessed its use as a potential diagnostic marker for the P status of photosynthetic picoplankton. Expression of PstS in Synechococcus sp. strain WH7803 was observed when the P1 concentration fell below 50 nM, demonstrating that the protein is induced at concentrations of P1 typical of oligotrophic conditions. PstS expression could be specifically detected by use of standard Western blotting (immunoblotting) techniques in natural mesocosm samples under conditions in which the N/P ratio was artificially manipulated to force P depletion. In addition, we have developed an immunofluorescence assay that can detect PstS expression in single Synechococcus cells both in laboratory cultures and natural samples. We show that antibodies raised against PstS cross-react with P-depleted Prochlorococcus cells, extending the use of these antibodies to both major groups of prokaryotic photosynthetic picoplankton. Furthermore, DNA sequencing of a Prochlorococcus pstS homolog demonstrated high amino acid sequence identity (77%) with the marine Synechococcus sp. strain WH7803 protein, including those residues in Escherichia coli PstS known to be directly involved in phosphate binding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.