ArticleJournal of bacteriology1997
Insertion mutagenesis of the lac repressor and its implications for structure-function analysis.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Ligand-specific changes in conformational flexibility mediate long-range allostery in the lac repressor.Nature communications · 2023Article
- Modular and Single-Cell Sensors of Bacterial Ser/Thr Kinase Activity.ACS synthetic biology · 2021Article
- A single mutation in the core domain of the lac repressor reduces leakiness.Microbial cell factories · 2013Article
- Transposon assisted gene insertion technology (TAGIT): a tool for generating fluorescent fusion proteins.PloS one · 2010Article
- High-resolution functional profiling of a gammaherpesvirus RTA locus in the context of the viral genome.Journal of virology · 2009Article
- High-resolution functional profiling of hepatitis C virus genome.PLoS pathogens · 2008Article
- TraY and integration host factor oriT binding sites and F conjugal transfer: sequence variations, but not altered spacing, are tolerated.Journal of bacteriology · 2007Article
- General mutagenesis of F plasmid TraI reveals its role in conjugative regulation.Journal of bacteriology · 2006Article
- Functional genomics on potato virus A: virus genome-wide map of sites essential for virus propagation.Genome research · 2002Article
- Analysis of functional domains of the Enterococcus faecalis pheromone-induced surface protein aggregation substance.Journal of bacteriology · 2001Article
- Exploring the role of integral membrane proteins in ATP-binding cassette transporters: analysis of a collection of MalG insertion mutants.Journal of bacteriology · 1998Article
- TnTIN and TnTAP: mini-transposons for site-specific proteolysis in vivo.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We recently developed a simple technique for the generation of relatively large (31-codon) insertion mutations in cloned genes. To test whether the analysis of such mutations could provide insight into structure-function relationships in proteins, we examined a set of insertion mutants of the Escherichia coli lac repressor (LacI). Representatives of several LacI mutant classes were recovered, including mutants which exhibit fully active, inducer-insensitive, or weak dominant-negative phenotypes. The various properties of the recovered mutants agree with previous biophysical, biochemical, and genetic data for the protein. In particular, the results support the prior designation of mutationally tolerant spacer regions of LacI as well as proposed differences in dimerization interactions among regions of the protein core domain. These findings suggest that the analysis of 31-codon insertion mutations may provide a simple approach for characterizing structure-function relationships in proteins for which high-resolution structures are not available.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.