ArticleJournal of bacteriology1997
Delineation of the interaction domains of Agrobacterium tumefaciens VirB7 and VirB9 by use of the yeast two-hybrid assay.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 54 citations in OpenAlex.
- Protein-Protein Interactions: Yeast Two Hybrid.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Protein interactions within and between two F-type type IV secretion systems.Molecular microbiology · 2020Article
- TraK and TraB are conserved outer membrane proteins of the Neisseria gonorrhoeae Type IV secretion system and are expressed at low levels in wild-type cells.Journal of bacteriology · 2014Article
- A component of the Xanthomonadaceae type IV secretion system combines a VirB7 motif with a N0 domain found in outer membrane transport proteins.PLoS pathogens · 2011Article
- The integrity of the periplasmic domain of the VirA sensor kinase is critical for optimal coordination of the virulence signal response in Agrobacterium tumefaciens.Journal of bacteriology · 2011Article
- The structural biology of type IV secretion systems.Nature reviews. Microbiology · 2009Review
- Agrobacterium VirB10 domain requirements for type IV secretion and T pilus biogenesis.Molecular microbiology · 2009Article
- Article
- Protein-protein interactions among Helicobacter pylori cag proteins.Journal of bacteriology · 2006Article
- Identification of new protein-protein interactions involving the products of the chromosome- and plasmid-encoded type IV secretion loci of the phytopathogen Xanthomonas axonopodis pv. citri.Journal of bacteriology · 2005Article
- Coccidioides posadasii contains a single 1,3-beta-glucan synthase gene that appears to be essential for growth.Eukaryotic cell · 2005Article
- Biogenesis, architecture, and function of bacterial type IV secretion systems.Annual review of microbiology · 2005Review
- Type IV secretion: the Agrobacterium VirB/D4 and related conjugation systems.Biochimica et biophysica acta · 2004Review
- Interaction between protein subunits of the type IV secretion system of Bartonella henselae.Journal of bacteriology · 2004Article
- VirB1 orthologs from Brucella suis and pKM101 complement defects of the lytic transglycosylase required for efficient type IV secretion from Agrobacterium tumefaciens.Journal of bacteriology · 2004Article
- The versatile bacterial type IV secretion systems.Nature reviews. Microbiology · 2003Review
- Conjugative coupling proteins interact with cognate and heterologous VirB10-like proteins while exhibiting specificity for cognate relaxosomes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Peptide linkage mapping of the Agrobacterium tumefaciens vir-encoded type IV secretion system reveals protein subassemblies.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Role of Agrobacterium VirB11 ATPase in T-pilus assembly and substrate selection.Journal of bacteriology · 2001Article
- VirB7 lipoprotein is exocellular and associates with the Agrobacterium tumefaciens T pilus.Journal of bacteriology · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Agrobacterium tumefaciens VirB proteins are postulated to form a transport pore for the transfer of T-DNA. Formation of the transport pore will involve interactions among the VirB proteins. A powerful genetic method to study protein-protein interaction is the yeast two-hybrid assay. To test whether this method can be used to study interactions among the VirB membrane proteins, we studied the interaction of VirB7 and VirB9 in yeast. We recently demonstrated that VirB7 and VirB9 form a protein complex linked by a disulfide bond between cysteine 24 of VirB7 and cysteine 262 of VirB9 (L. Anderson, A. Hertzel, and A. Das, Proc. Natl. Acad. Sci. USA 93:8889-8894, 1996). We now demonstrate that VirB7 and VirB9 interact in yeast, and this interaction does not require the cysteine residues essential for the disulfide linkage. By using defined segments in fusion constructions, we mapped the VirB7 interaction domain of VirB9 to residues 173 to 275. In tumor formation assays, both virB7C24S and virB9C262S expressed from a multicopy plasmid complemented the respective deletion mutation, indicating that the cysteine residues may not be essential for DNA transfer.
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