ArticleThe EMBO journal1997
Histone octamer function in vivo: mutations in the dimer-tetramer interfaces disrupt both gene activation and repression.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 88 citations in OpenAlex.
- Molecular and structural basis of the chromatin remodeling activity by Arabidopsis DDM1.Nature communications · 2024Article
- A novel histone H4 variant H4G regulates rDNA transcription in breast cancer.Nucleic acids research · 2019Article
- The histone chaperone FACT modulates nucleosome structure by tethering its components.Life science alliance · 2018Article
- The conformational flexibility of the C-terminus of histone H4 promotes histone octamer and nucleosome stability and yeast viability.Epigenetics & chromatin · 2012Article
- Chromatin and transcription in yeast.Genetics · 2012Review
- Role of histone tails in structural stability of the nucleosome.PLoS computational biology · 2011Article
- Human linker histones: interplay between phosphorylation and O-β-GlcNAc to mediate chromatin structural modifications.Cell division · 2011Article
- A conserved patch near the C terminus of histone H4 is required for genome stability in budding yeast.Molecular and cellular biology · 2011Article
- Histone tyrosine phosphorylation comes of age.Epigenetics · 2011Review
- Article
- FACT prevents the accumulation of free histones evicted from transcribed chromatin and a subsequent cell cycle delay in G1.PLoS genetics · 2010Article
- Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis.Nature cell biology · 2009Article
- Saccharomyces cerevisiae linker histone Hho1p functionally interacts with core histone H4 and negatively regulates the establishment of transcriptionally silent chromatin.The Journal of biological chemistry · 2009Article
- Dispersed mutations in histone H3 that affect transcriptional repression and chromatin structure of the CHA1 promoter in Saccharomyces cerevisiae.Eukaryotic cell · 2008Article
- Multiscale modeling of nucleosome dynamics.Biophysical journal · 2007Article
- Article
- The tale beyond the tail: histone core domain modifications and the regulation of chromatin structure.Nucleic acids research · 2006Review
- High-resolution structure of the native histone octamer.Acta crystallographica. Section F, Structural biology and crystallization communications · 2005Article
- Histone H4 lysine 91 acetylation a core domain modification associated with chromatin assembly.Molecular cell · 2005Article
- Mutations in the nucleosome core enhance transcriptional silencing.Molecular and cellular biology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Within the core histone octamer each histone H4 interacts with each H2A-H2B dimer subunit through two binding surfaces. Tyrosines play a central role in these interactions with H4 tyrosines 72 and 88 contacting one H2A-H2B dimer subunit, and tyrosine 98 contacting the other. To investigate the roles of these interactions in vivo, we made site-directed amino acid substitutions at each of these tyrosine residues. Elimination of either set of interactions is lethal, suggesting that binding of the tetramer to both dimers is essential. Temperature-sensitive mutants were obtained through single amino acid substitutions at each of the tyrosines. The mutants show both strong positive and negative effects on transcription. Positive effects include Spt- and Sin-phenotypes resulting from mutations at each of the three tyrosines. One allele has a strong negative effect on the expression of genes essential for the G1 cell cycle transition. At restrictive temperature, mutant cells fail to express the CLN1, CLN2, SWI4 and SWI6 genes, and have reduced levels of CLN3 mRNA. These results demonstrate the critical role of histone dimer-tetramer interactions in vivo, and define their essential role in the expression of genes regulating G1 cell cycle progression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.