ArticleThe EMBO journal1997
Translocation of the Csk homologous kinase (Chk/Hyl) controls activity of CD36-anchored Lyn tyrosine kinase in thrombin-stimulated platelets.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 74 citations in OpenAlex.
- "Lyn"king Emphysema and Cancer Development: Insights from Src Family Kinase Gain-of-Function Models.American journal of respiratory cell and molecular biology · 2023Article
- Regulation, targets and functions of CHK.Frontiers in cell and developmental biology · 2022Review
- Article
- Article
- C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src.Molecular biology of the cell · 2018Article
- Review
- Phosphorylation of KRAB-associated protein 1 (KAP1) at Tyr-449, Tyr-458, and Tyr-517 by nuclear tyrosine kinases inhibits the association of KAP1 and heterochromatin protein 1α (HP1α) with heterochromatin.The Journal of biological chemistry · 2013Article
- Functions of the Lyn tyrosine kinase in health and disease.Cell communication and signaling : CCS · 2012Article
- CD36 protein is involved in store-operated calcium flux, phospholipase A2 activation, and production of prostaglandin E2.The Journal of biological chemistry · 2011Article
- Article
- Regulation of platelet function by class B scavenger receptors in hyperlipidemia.Arteriosclerosis, thrombosis, and vascular biology · 2010Review
- The Y's that bind: negative regulators of Src family kinase activity in platelets.Journal of thrombosis and haemostasis : JTH · 2009Review
- Decreased CHK protein levels are associated with Src activation in colon cancer cells.Oncogene · 2008Article
- Paxillin family members function as Csk-binding proteins that regulate Lyn activity in human and murine platelets.The Biochemical journal · 2007Article
- Trafficking of Lyn through the Golgi caveolin involves the charged residues on alphaE and alphaI helices in the kinase domain.The Journal of cell biology · 2004Article
- Identification of a suppressor of the Dictyostelium profilin-minus phenotype as a CD36/LIMP-II homologue.The Journal of cell biology · 1999Article
- Physical and functional association of the Src family kinases Fyn and Lyn with the collagen receptor glycoprotein VI-Fc receptor gamma chain complex on human platelets.The Journal of experimental medicine · 1998Article
- Active Lyn protein tyrosine kinase is selectively enriched within membrane microdomains of resting platelets.The Biochemical journal · 1998Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chk/Hyl is a recently isolated non-receptor tyrosine kinase with greatest homology to a ubiquitous negative regulator of Src family kinases, Csk. To understand the significance of co-expression of Chk and Csk in platelets, we examined the subcellular localization of each protein. Chk, but not Csk, was completely translocated from the Triton X-100-soluble to the Triton X-100-insoluble cytoskeletal fraction within 10 s of thrombin stimulation. Chk and Lyn, but not Csk and c-Src, co-fractionated in the higher density lysate fractions of resting platelets, with Chk being found to localize close to CD36 (membrane glycoprotein IV)-anchored Lyn. The kinase activity of co-fractionated Lyn was suppressed 3-fold. In vitro phosphorylation assays showed that Chk suppressed Lyn activity by phosphorylating its C-terminal negative regulatory tyrosine. Upon stimulation of platelets with thrombin, the rapid and complete translocation of Chk away from Lyn caused concomitant activation of Lyn. This activation was accompanied by dephosphorylation of Lyn at its C-terminal negative regulatory tyrosine in cooperation with a protein tyrosine phosphatase. These results suggest that Chk, but not Csk, may function as a translocation-controlled negative regulator of CD36-anchored Lyn in thrombin-induced platelet activation.
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